Rat IL34 Gene cDNA Clone / ORF Clone

IL34 cDNA Clone Datasheet

IL34 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001025766.1
• cDNA Size: 705
• Gene Synonym: MGC112852, Il34
• Source: Rat

IL34 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 34 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 241-243 CAG insertion mutation.

IL34 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL34 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL34 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCCTGGG GACTCGCCTG GTTATACTGT CTTGGGATCC TACTTGACGT GGCTTTGGGA
61       AACGAGAATT TGGAGATATG GACTCTGGCC CAAGATAAAG AATGTGATCT TACAGGCTAC
121      CTTCGGGGCA AGCTGCAGTA CAAGAACCGG CTTCAGTACA TGAAACATTA CTTCCCCATC
181      AACTACAGGA TTGCTGTGCC TTATGAGGGG GTACTCAGAG TGGCCAACAT CACGAGGCTG
241      CAGAAGGCCC ATGTGAGTGA GCGAGAGCTG CGGTACCTGT GGGTCTTGGT GAGCCTCAAC
301      GCCACTGAGT CTGTGCTGGA TGTGCTGCTG GAGGGACACC CATCCTGGAA GTATCTACAG
361      GAGGTTCAGA CATTGCTGGA GAATGTTCAG CGGAGCCTCA TGGATGTGGA GATTGGCCCT
421      CACGTGGAAG CTGTGTTATC TCTTCTGAGT ACCCCTGGCC TAAGCCTGAA GCTGGTGCGG
481      CCCAAAGCCT TGTTGGATAA CTGCTTCCGG GTCATGGAGC TGCTGTACTG CTCTTGCTGC
541      AAACAAAGTC CCATCTTAAA ATGGCAGGAC TGTGAGCTGC CCAGGCTCCA TCCCCACAGT
601      CCAGAGTCCT TGATGCAATG TGCAGCTACC AACGTGTACC CTTTGCCTCG GCAGCCCCCC
661      ACCTCCCTGC CCAGGTCCCC AAGTTCAAAC CATGGCCCGT TGCCCTGA

 

Quality Control:cDNA sequencing results is available upon request.