Human IL2RG ORF Expression Clone, FLAG-tagged (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG10555-M-F	pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL2RG cDNA Clone Datasheet

IL2RG cDNA Clone Product Information

Gene Bank Ref.ID:	NM_000206.1
cDNA Size:	1137
Gene Synonym:	IMD4; CD132; SCIDX; SCIDX1
Source:	Human

IL2RG cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens interleukin 2 receptor, gamma (severe combined immunodeficiency) DNA.
Vector:	pCMV/hygro-FLAG
FLAG Tag Sequence:	GATTACAAGGATGACGACGATAAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence.

IL2RG cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL2RG cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL2RG cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTGAAGC CATCATTACC ATTCACATCC CTCTTATTCC TGCAGCTGCC CCTGCTGGGA
61       GTGGGGCTGA ACACGACAAT TCTGACGCCC AATGGGAATG AAGACACCAC AGCTGATTTC
121      TTCCTGACCA CTATGCCCAC TGACTCCCTC AGTGTTTCCA CTCTGCCCCT CCCAGAGGTT
181      CAGTGTTTTG TGTTCAATGT CGAGTACATG AATTGCACTT GGAACAGCAG CTCTGAGCCC
241      CAGCCTACCA ACCTCACTCT GCATTATTGG TACAAGAACT CGGATAATGA TAAAGTCCAG
301      AAGTGCAGCC ACTATCTATT CTCTGAAGAA ATCACTTCTG GCTGTCAGTT GCAAAAAAAG
361      GAGATCCACC TCTACCAAAC ATTTGTTGTT CAGCTCCAGG ACCCACGGGA ACCCAGGAGA
421      CAGGCCACAC AGATGCTAAA ACTGCAGAAT CTGGTGATCC CCTGGGCTCC AGAGAACCTA
481      ACACTTCACA AACTGAGTGA ATCCCAGCTA GAACTGAACT GGAACAACAG ATTCTTGAAC
541      CACTGTTTGG AGCACTTGGT GCAGTACCGG ACTGACTGGG ACCACAGCTG GACTGAACAA
601      TCAGTGGATT ATAGACATAA GTTCTCCTTG CCTAGTGTGG ATGGGCAGAA ACGCTACACG
661      TTTCGTGTTC GGAGCCGCTT TAACCCACTC TGTGGAAGTG CTCAGCATTG GAGTGAATGG
721      AGCCACCCAA TCCACTGGGG GAGCAATACT TCAAAAGAGA ATCCTTTCCT GTTTGCATTG
781      GAAGCCGTGG TTATCTCTGT TGGCTCCATG GGATTGATTA TCAGCCTTCT CTGTGTGTAT
841      TTCTGGCTGG AACGGACGAT GCCCCGAATT CCCACCCTGA AGAACCTAGA GGATCTTGTT
901      ACTGAATACC ACGGGAACTT TTCGGCCTGG AGTGGTGTGT CTAAGGGACT GGCTGAGAGT
961      CTGCAGCCAG ACTACAGTGA ACGACTCTGC CTCGTCAGTG AGATTCCCCC AAAAGGAGGG
1021     GCCCTTGGGG AGGGGCCTGG GGCCTCCCCA TGCAACCAGC ATAGCCCCTA CTGGGCCCCC
1081     CCATGTTACA CCCTAAAGCC TGAAACCGCT¹ GATTACAAGG ATGACGACGA TAAG²TAA

1. GCT was the nucleotide residue from the restriction site during plasmid construction,
which has no influence on protein expression.
2. FLAG Tag

Quality Control:cDNA sequencing results is available upon request.

Cytokine