Rat IL2RB Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80340-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL2RB cDNA Clone Datasheet

IL2RB cDNA Clone Product Information

Gene Bank Ref.ID:	NM_013195.1
cDNA Size:	1614
Gene Synonym:	IL2RBC; Il2rb
Source:	Rat

IL2RB cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus interleukin 2 receptor, beta DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 645 T/A not causing the amino acid variation.

IL2RB cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL2RB cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL2RB cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCACCG TAGACCTTTC GTGGCGTCTG CCCCTCTACA TCCTCCTCTT GCTCCTGGCT
61       ACAACTTGGG TGTCTGCAGC AGTGAACGAC TGTTCCCATC TTAAGTGCTT CTACAACTCA
121      AGAGCCAATG TCTCCTGCAT GTGGAGCCCT GAAGAGGCCC TGAACGTCAC ATCCTGTCAC
181      ATCCATGCCA AGTCGGATAT GCGACACTGG AATAAAACCT GTGAGCTAAC CCCTGTGAGG
241      CAGGCATCCT GGGCCTGCAA CCTGATTCTC GGGCCCCTCC CAGATTCCCA GTCACTGACC
301      TCCGTAGACC TCCTCAGCCT AAGTGTGGTG TGCTGGGAAG AGAAGGGGTG GCGTAGGGTG
361      AAGACCTGCA CCTTCCATCC CTTTGACAAC CTTCGCCTGA TTGCCCCTCA TTCCCTACAA
421      GTCCTGCACA TTGAAACCCG GAGATGTAAC ATAAGCTGGG AGGTGTCCCA GGTCTCTCAC
481      TACGTTAATC CATACTTGGA ATTTGAGGCC CGTAGACGTC TTCTGGACCG TAGCTGGGAG
541      GATGCATCCG TATTCAGCCT CAAGCAGAGG CAGCAGTGGA TCTTCTTGGA GACGCTGACC
601      CCTGACACCT CATATGAGCT CCAGGTGAGG GTCATAGCTC AACGCGGCAA AACCAGGACC
661      TGGAGCCCCT GGAGCCAGCC CATGGCCTTT CGGACAAGGC CAGCAGATCC TAAGGAGATC
721      TTCCCCCTGC CATGGCTCAG ATGCCTTCTA CTGGTCCTCG GCTGCTTCTT TGGCTTCTTG
781      TCCTGCGTCT GCGTTTTGGT CAAGTGCCGC TACCTTGGGC CATGGCTGAA GACACTTCTC
841      AAGTGCCACA TCCCAGATCC GTCTGAGTTC TTCTCCCAAC TGAGCTCCCA GCATGGGGGA
901      GACCTTCAGA AATGGCTCTC CTCGCCTGTC CCCCAATCCT TCTTCAGCCC CACTGGCTCT
961      GCACCTGAGA TCTCCCCACT GGAAGTGCTT GACAGAGATT CCAAGACCAT GCAGATGCTC
1021     CTGTTTCAGA AGGAGAAGGC CTCTTCACCC TCGCCCAGTG GCCACTCACA GGCCAGCTGC
1081     TTCACCAACC AAGGTTACTT CTTCTTCCAT TTGTCCAATG CCTTGGAGAT CGAGTCCTGC
1141     CAGGTGTACT TCACCTATGA CCCCTGCATG GAGGAGGATG TGGAGGAAGA TGGGCCAAGG
1201     CTGCCCGAGG AATCTCCCCT CCCACCTCTG CTGCCTTTCA CTGGAGAACA GGATGACTAC
1261     TGTGCCTTCC CGCCCAGGGA TGACCTGCTG CTCTTCTCCC CAAGCATGAG CACCCCCAAC
1321     ACTGCCTATG GGAACAGCAT AACCCCGGAA GAAAGGCCTC CCCTCTCCCT GCAGGAGGGG
1381     CTGCCCTCCC TAGCATCCCC TGACCTGATG GGCTTACAGC ACCCTCTGGA GCTGGAGCTG
1441     GGAGATGATG GAGAAGGGAT GTCTACCAAT AGCTCTGGGC AACAGGCCAG TGTCCCGGAA
1501     GCAGCTCTCA TGGGCACGAC CAAGACAGAG GCCAGGCCTG TCCTGACTCT AAACACTGAT
1561     GCCTATCTGT CTCTTCAAGA ACTACAGGCC CAAGATTCAG CCCACCTAAT ATAG

Quality Control:cDNA sequencing results is available upon request.

CD122/IL-2RB related areas, pathways, and other information

Cytokine