Mouse IL27 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG51107-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL27 cDNA Clone Datasheet

IL27 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_145636.1
cDNA Size:	705
Gene Synonym:	p28; Il30; IL-27; IL-27p28
Source:	Mouse

IL27 cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus interleukin 27 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

IL27 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL27 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL27 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCCAGG TGACAGGAGA CCTTGGCTGG CGGCTCAGCC TGTTGCTGCT ACCCTTGCTT
61       CTGGTACAAG CTGGTTCCTG GGGGTTCCCA ACAGACCCCC TGAGCCTTCA AGAGCTGCGC
121      AGGGAATTCA CAGTCAGCCT GTACCTTGCC AGGAAGCTGC TCTCTGAGGT TCAGGGCTAT
181      GTCCACAGCT TTGCTGAATC TCGATTGCCA GGAGTGAACC TGGACCTCCT GCCCCTGGGA
241      TACCATCTTC CCAATGTTTC CCTGACTTTC CAGGCATGGC ATCACCTCTC TGACTCTGAG
301      AGACTCTGCT TCCTCGCTAC CACACTTCGG CCCTTCCCTG CCATGCTGGG AGGGCTGGGG
361      ACCCAGGGGA CCTGGACCAG CTCAGAGAGG GAGCAGCTGT GGGCCATGAG GCTGGATCTC
421      CGGGACCTGC ACAGGCACCT CCGCTTTCAG GTGCTGGCTG CAGGATTCAA ATGTTCAAAG
481      GAGGAGGAGG ACAAGGAGGA AGAGGAAGAG GAGGAAGAAG AAGAAAAGAA GCTGCCCCTA
541      GGGGCTCTGG GTGGCCCCAA TCAGGTGTCA TCCCAAGTGT CCTGGCCCCA GCTGCTCTAT
601      ACCTACCAGC TCCTTCACTC CCTGGAGCTT GTCCTGTCTC GGGCTGTTCG GGACCTGCTG
661      CTGCTGTCCC TGCCCAGGCG CCCAGGCTCA GCCTGGGATT CCTAA

Quality Control:cDNA sequencing results is available upon request.

Cytokine