Rat IL27 Gene cDNA Clone / ORF Clone

IL27 cDNA Clone Datasheet

IL27 cDNA Clone Product Information

• Gene Bank Ref.ID: XM_344962.4
• cDNA Size: 705
• Gene Synonym: RGD1561420, Il27
• Source: Rat

IL27 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 27 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 465 A/C not causing the amino acid variation.

IL27 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL27 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL27 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCCAGG TGACAGGAGG CCTTGGCTGG CGGCTCAGCC TGTTGCTGCT ACCTTTGCTT
61       ATGGTACAAA CTGGTTCCTG GGGGTTCCCA GCAGACCCCC TGAGCCTTCA AGAGCTGCGC
121      AGGGAATTCA CAGTCAGCCT GTACCTTGCC AGGAAACTGC TCTCTGAGGT TCAGGGCTAT
181      GTCCACAGTT TTGCTGAATC TCGCTTGCCA GGAGTGAACC TGGACCTCCT GCCCGTGGGA
241      CACCATCTCC CCAATGTTTC CCTGACCTTC CAGGCATGGC GTCACCTCTC TGACTCTGAC
301      AGACTCTGCT TCCTCGCTAC CACACTTCGA CCCTTCCCTG CTCTGCTGGG AGGGCTGGAG
361      ACCCAGAGGA CCTGGACCAG CTCAGAGAGG GAGCAGCTGT GGGCAATGAG GCTGGATCTC
421      CGGGACCTGC ACAGGCACCT CCGCTTCCAG GTGCTGGCTG TAGGCTTCAG CTGTTCAGAG
481      GAGGAAAAGG AAGAGGAAGA AGAGGACGAG GAGGAGGAGG AAGGGAAGGA GCTGCTCCTA
541      GGGGCTCTGG GTGGCCCCAA CCAGGTGTCA TCCCAAGTGT CCTGGCCCCA GCTGCTCTAT
601      GCTTACCAGC TCCTACATTC CCTGGAGCTT GTCCTATCTC GGGCTGTTCG GGACCTGCTG
661      CTGTTGTCCT TGCCCAGGCG TCCAGACTCA GCTTGTGATC CCTAA

 

Quality Control:cDNA sequencing results is available upon request.