Rat IL23R Gene cDNA Clone / ORF Clone (Cytokine)

IL23R cDNA Clone Datasheet

IL23R cDNA Clone Product Information

• Gene Bank Ref.ID: XM_002726387.1
• cDNA Size: 1920
• Gene Synonym: Il23r
• Source: Rat

IL23R cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 23 receptor DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 368 G/A resulting in the amino acid Arg substitution by His.

IL23R cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL23R cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL23R cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAAGAG AGAGAGAAAT GAGAGGTTTC TACTACATTT GGGACATGAG TCACGTCGCA
61       CTTCAGTTGC ACGTGGTAAT AGCCCTTTAT GCGCTCTTCA GATGGGGTCA TGGAGGAATT
121      GCAACTATAA ACTGCTCTGG TAATATGTGG GTTGAGCCTG GTGAAATTTT TCAGATGGGT
181      ATGAATGTTT CTGTATATTG CCAAGAAGCT CTTAAGAACT GCCGACCAAG GAATCTTCAC
241      TTTTATAAAA ATGGCTTCAA AGAAAGATTT CATATCACAA GGATTAATAG AACAACAGCT
301      CGAGTTTGGT ATAAAGGCTT TTCGGAACCT CATGCCTCTA TGTATTGCAC TGCTGAATGT
361      CCTGGGCATT TTCAAGAGAC ACTCATTTGT GGGAAAGACA TTTCCTCTGG ATATCCACCA
421      GATGCCCCCA GCAATATGAC ATGTGTCATT TATGAATACT CAGGCAACAT GACTTGCACC
481      TGGAACACTG GGAAGCCCAC CTACATAGAT ACCAAGTATA CTGTGCACGT GAAGAGTTTG
541      GAGACAGAAG AACAACAACA ATATCTTGCC TCAAACTATG TTAACATCTC CACTGACTCA
601      TTGCAAGGTG GCAGAAAGTA TCTGGTATGG GTCCAAGCTG TCAATGCCCT AGGCATGGAG
661      AACTCACAAC AACTACAAGT TCATCTGGAT GATATAGTGA TACCTTCTCC ATCCATCATT
721      TCCAGGGCTG AGACTACAAA TGCTAATGTG CCCAAGACCA TAATTTACTG GAAGAGCAAA
781      ATTATGACTG GGAAAGTATT CTGTGAGATG AGATACAAAG CAACAACAAA CCAAACATGG
841      AATGTTAAAG AATTTGACAC CAATTACACA TATGTGCAGC AGTCAGAATT CTACCTGGAG
901      CCAAACAGCA AGTATGTATT TCAAGTGAGA TGTCAAGGAA CTGGTAAAAG AAACTGGCAG
961      CCTTGGAGCT CGCCCTTTGT CCACCAAACT CCCCAGACAG CTTCCCAGGT TACATCAAAA
1021     CCACCCCACG AACCTCAGAA GATTGAGATG CTCACTGCTA CAATCTTCAA AGGACATTCT
1081     ACTTCAGATA ATAGTCAAGA CATTGGACTT TTGTCGGGAA TGGTCTTCTT GGCCATCTTG
1141     TTGCCGATTT TTTCTCTGAT TGGGATATTT AACAGATCAC TTCGAATAGG AATTAAAAGA
1201     AAAGTCTTAC TGATGATCCC AAAGTGGCTT TATGAAGATA TTCCCAATAT GGAAAATAGT
1261     AATATTGCAA AATTATTACA GGAAAAAAGT GTATTTGAAA ACGAAAATGC CAGCGAGCAG
1321     GCCCTGTATG TGGATCCTAT CCTTACAGAG ATAAGTGAAA TCTCTCCCCT GGAACACAAA
1381     CCCACAGATT ACAAAAAAGA CAGGCTCACA GGATTCCTTG AGACAAGAGA CTGTCCACTA
1441     ACAACATTGT CTACCAGTTC TTCTGTTGTG TATATTCCTG ATCTCAACAC TGGATACAAA
1501     CCCCAGGTTT CAAATGTACC CCCTGAGGAA AACCATTTCA TTAATAGAGA TGAAAGAGAC
1561     CCTATGTCCC TTGAGGCCAC AGGTGACCAC TTTGCCAGAT TGAAAACATA TCCCAACTTC
1621     ACATTTTCTG CTTCAAGTAT GACTTCACTA AGCAAAACAC TAATTCTTGA TGAATTGAGC
1681     CTCGTTTTAA ATCAAGGAGA ATTCAATTCT CTTGACATAC AAAACTCAAG ACAGGAGGAA
1741     ACCAGCATGA TTTTGCAAAA TGACTCACCC AGTGAAACTA TCCCAGTGCA GACTCTGCTG
1801     CCTGATGAAT TTGTCTCCTG TTTGGCAATC GGGAGTGAAG ACTTGCCGTC TATTAACTCT
1861     TACTTTCCAC AGAACGTTTT GGAAAGCCAT TTCAGTGGGA TTCCACTCTT GCAAAAGTAG

 

Quality Control:cDNA sequencing results is available upon request.