Mouse IL22 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG51085-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL22 cDNA Clone Datasheet

IL22 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_016971.1
cDNA Size:	540
Gene Synonym:	IL-22; Iltif; IL-TIF
Source:	Mouse

IL22 cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus interleukin 22 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 251 G/A resulting in the amino acid Met substitution by Ile and 150 A/G not causing the amino acid variation.

IL22 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL22 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL22 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGTCC TGCAGAAATC TATGAGTTTT TCCCTTATGG GGACTTTGGC CGCCAGCTGC
61       CTGCTTCTCA TTGCCCTGTG GGCCCAGGAG GCAAATGCGC TGCCCGTCAA CACCCGGTGC
121      AAGCTTGAGG TGTCCAACTT CCAGCAGCCG TACATCGTCA ACCGCACCTT TATGCTGGCC
181      AAGGAGGCCA GCCTTGCAGA TAACAACACA GATGTCCGGC TCATCGGGGA GAAACTGTTC
241      CGAGGAGTCA ATGCTAAGGA TCAGTGCTAC CTGATGAAGC AGGTGCTCAA CTTCACCCTG
301      GAAGACGTTC TGCTCCCCCA GTCAGACAGG TTCCAGCCCT ACATGCAGGA GGTGGTGCCT
361      TTCCTGACCA AACTCAGCAA TCAGCTCAGC TCCTGTCACA TCAGCGGTGA CGACCAGAAC
421      ATCCAGAAGA ATGTCAGAAG GCTGAAGGAG ACAGTGAAAA AGCTTGGAGA GAGTGGAGAG
481      ATCAAGGCGA TTGGGGAACT GGACCTGCTG TTTATGTCTC TGAGAAATGC TTGCGTCTGA

Quality Control:cDNA sequencing results is available upon request.

Cytokine