Canine IL21 Gene cDNA Clone / ORF Clone (Cytokine)

IL21 cDNA Clone Datasheet

IL21 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001003347.1
• cDNA Size: 441
• Gene Synonym: IL-21;IL21
• Source: Canine

IL21 cDNA Clone Description

• cDNA Description: ORF Clone of Canis lupus familiaris interleukin 21 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

IL21 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL21 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL21 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGAAGA TAGTCATCTG CCTGATGGTC ATCTTCTTGG GGACAGTGGC CCATAAATCA
61       AGCTTCCAAG AGCAAGATCT TCTCTTGATT AGAATGCGTC AACTTATAGA TATTGTTGAT
121      CAGCTTAAAA ATTATGTGAA TGACTTGGAC CCTGAATCTC TGCCAGCTCC AGAAGATGTA
181      AAGAGGCATT GTGAGCGGTC AGCTTTTTCA TGTTTTCAGA AGGTCCAACT AAAGGCAGCA
241      AACACAGGAG GCAATGAACA GATAATCAAT GTATTAACTA AACAGCTGAA GAGGAAACTA
301      CCTCCCACAA ATGCAGGCAG AAGACAGAAA CACAGACCAG CATGCCCCTC ATGTGATTCT
361      TATGAGAAAG CACCACCCAA AGAATTCCTA GAAAGACTGA AATCACTCAT CCAAAAGATG
421      ATTCATCAAC ATCTCTCCTA G

 

Quality Control:cDNA sequencing results is available upon request.