Rat IL21 Gene cDNA Clone / ORF Clone

IL21 cDNA Clone Datasheet

IL21 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001108943.2
• cDNA Size: 441
• Gene Synonym: IL-21, Il21
• Source: Rat

IL21 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 21 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

IL21 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL21 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL21 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGAGAGGA CCCTTGTCTG TCTGATACTC ATCTTCTTGG GGACCGTGGC CCATAAATCA
61       AGCCCCCAAA GGCCAGATCA CCTTCTGATT AGACTTCGTC ACCTTATGGA CATTGTTGAA
121      CAGCTGAAAA TCTATGAGAA TGACTTGGAT CCTGAACTTC TAACAGCTCC ACAAGATGTA
181      AAGGGGCAAT GTGAGCACGA AGCTTTTGCC TGTTTTCAGA AAGCCAAACT CAAGCCATCA
241      AACACTGGAA ACAATAAGAC ATTCATCAAC GACTTGTTGG CACAGCTGAG GAGGAGGCTG
301      CCTGCTAAGA GGACAGGAAA TAAACAGAGG CACATGGCTA AATGCCCTTC CTGTGATTTG
361      TATGAGAAAA AAACACCCAA AGAATTCCTT GAAAGACTAA AATGGCTCCT TCAAAAGATG
421      ATTCATCAGC ATCTTTCCTA G

 

Quality Control:cDNA sequencing results is available upon request.