Mouse IL21 / Interleukin 21 Protein (Native) (Cytokine)

Interleukin 21 Protein Datasheet

IL21 / Interleukin 21 Protein Product Information

• Synonym: DN-452A22.7
• Protein Construction: A DNA sequence encoding the soluble form of mouse IL21 (Q9ES17) (Pro 25-Ser 146) was expressed, with an initial Met at the C-terminus.
• Source: Mouse
• Expression Host: E.coli

IL21 / Interleukin 21 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse IL21 consists of 123 amino acids and has a calculated molecular mass of 14.3KDa. It migrates as a 15 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH 7.5.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL21 / Interleukin 21 protein

IL21 / Interleukin 21 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL21 / Interleukin 21 Protein Description

IL21 is a cytokine with immunoregulatory activity. It is a member of the IL-15/IL-21 family. It is expressed in activated CD4-positive T-cells but not in CD8-positive T-cells, B-cells, or monocytes. IL21 may promote the transition between innate and adaptive immunity. It induces the production of IgG(1) and IgG(3) in B-cells (By similarity). IL21 may also play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. The proliferation of mature B- and T-cells in response to activating stimuli may be regulated by IL21. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells. During T-cell mediated immune response it may inhibit dendritic cells (DC) activation and maturation. Systemic treatment of IL-21 may be an effective means of mitigating the allergic response. IL-21 also may be a critical factor in the control of persistent viral infections.

References

1. Coquet JM. et al., 2007, J Immunol. 178 (5): 2827-34.
2. Wei L. et al., 2007, J Biol Chem. 282 (48): 34605-10.
3. Parrish-Novak J. et al., 2002, J Leukoc Biol. 72 (5): 856-63.
4. Kuchen S. et al., 2007, J Immunol. 179 (9): 5886-96.