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Rat IL20 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog Vector (Price) Quantity In Stock Operation
RG80422-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL20 cDNA Clone Datasheet

 

IL20 cDNA Clone Product Information

Gene Bank Ref.ID: NM_001143881.1

cDNA Size:

531

Gene Synonym:

Il20

Source: Rat

IL20 cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus interleukin 20 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref.ID sequence.

IL20 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL20 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL20 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAGGCT TTCGTCTTGC CTTTGGACTG TTCTCCGTTG TGGGTTTTCT TCTCTGGACT
61       CCTTTAACTG GGCTCAAGAC CCTTCATTTG GGAAGCTGTG TAATCACTGC AAACCTACAG
121      GCGATACAAA AGGAATTTTC TGAGATTCGG CATAGTGTGC AAGCTGAAGA TGAAAATATC
181      GACGTCAGGA TTTTAAGGAC GACTGAGTCC CTGAAAGACA CAAAGCTTTC GGATAGGTGC
241      TGCTTTCTCC GCCATCTAGT GAGGTTCTAT CTGGACAGGG TGTTCAAAGT CTACCAGACC
301      CCTGACCATC ATACCCTGCG AAAGATCAGC AGCCTCGCCA ATTCTTTTCT TATCATCAAG
361      AAGGACCTCT CAGTCTGTCA TTCTCACATG GCATGTCATT GTGGCGAAGA AGCAATGGAG
421      AAATACAACC AAATTCTCAG TCATTTCACA GAGCTTGAGC TCCAGGCAGC CGTGGTGAAG
481      GCTTTGGGGG AACTAGGCAT TCTTCTGAGA TGGATGGAGG AGATGTTATA G

 

Quality Control:cDNA sequencing results is available upon request.