Canine IL2 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
DG70014-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL2 cDNA Clone Datasheet

IL2 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001003305.1
cDNA Size:	468
Gene Synonym:	IL2
Source:	Canine

IL2 cDNA Clone Description

cDNA Description:	ORF Clone of Canis lupus familiaris interleukin 2 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 34 C/T not causing the amino acid variation.

IL2 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL2 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTACAAAA TGCAACTCTT GTCTTGCATC GCATTGACGC TTGTACTTGT CGCAAACAGT
61       GCACCTATTA CTTCAAGCTC TACAAAGGAA ACAGAGCAAC AGATGGAGCA ATTACTGCTG
121      GATTTACAGT TGCTTTTGAA TGGAGTTAAT AATTATGAGA ACCCCCAACT CTCCAGGATG
181      CTCACATTTA AGTTTTACAC GCCCAAGAAG GCCACAGAAT TTACACACCT TCAATGTCTA
241      GCAGAAGAAC TCAAAAACCT GGAGGAAGTG CTAGGTTTAC CTCAAAGCAA AAACGTTCAC
301      TTGACAGACA CCAAGGAATT AATCAGCAAT ATGAATGTAA CACTTCTGAA ACTAAAGGGA
361      TCTGAAACAA GTTACAACTG TGAATATGAT GACGAGACAG CAACCATTAC AGAATTTCTG
421      AACAAATGGA TTACCTTTTG TCAAAGCATC TTCTCAACAC TGACTTGA

Quality Control:cDNA sequencing results is available upon request.

Cytokine