Rat IL1R2 Gene cDNA Clone / ORF Clone

IL1R2 cDNA Clone Datasheet

IL1R2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_053953.1
• cDNA Size: 1251
• Gene Synonym: Il-1r2, Il1rb, Il1r2
• Source: Rat

IL1R2 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 1 receptor, type II DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 996 A/G not causing the amino acid variation.

IL1R2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL1R2 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL1R2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTCATCT TGCTTGTGTT AGTAACTGGG GTTTCAGCTT TCACCACTCC AGCAGTGGTG
61       CACACAGGAA GGGTTTCTGA GTCTCCCGTT ACATCAGAGA AGCACCCAGT TCTTGGAGAC
121      GATTGCTGGT TTCGTGGCAG AGATTTCAAA TCGGAATTGA GGCTGGAAGG TGAGCCCGTG
181      GTCCTGAGGT GCCCCCTGGT ACCTCATTCA GACACCTCCA GCAGTTCCCG CAGCCTTCTG
241      ACATGGAGTA AATCGGACTC TTCTCAGCTG ATCCCAGGAG ATGAGCCAAG GATGTGGGTG
301      AAGGATGATA CGCTCTGGGT TCTGCCAGCA GTTCAGCAGG ACTCTGGTAC CTACATCTGC
361      ACATTCAGAA ACGCGTCCCA CTGTGAACAA ATGTCTCTGG AACTCAAGGT CTTTAAGAAT
421      ACCGAAGCCT CTTTCCCTCT CGTCTCCTAC TTGCAAATCT CAGCTCTCTC CTCCACCGGG
481      TTACTTGTGT GCCCTGACCT GAAAGAGTTC ATCTCCAGCA GAACTGATGG AAAGATACAG
541      TGGTATAAGG GCTCCATCCT CTTGGATAAA GGCAATAAGA AATTTCTGAG TGCGGGAGAC
601      CCCACACGCC TATTGATATC CAACACATCC ATGGGAGATG CAGGCTATTA CAGATGTGTT
661      ATGACATTTA CCTACGAGGG CAAGGAATAC AACATCACTA GGAATATTGA ACTCCGGGTC
721      AAAGGAATAA CCACAGAACC CATCCCTGTG ATCATTTCTC CCCTGGAGAC AATACCAGCA
781      TCTTTGGGGT CAAGACTGAT AGTCCCGTGC AAGGTGTTCC TGGGAACTGG TACATCTTCC
841      AACACCATCG TGTGGTGGAT GGCTAACAGC ACGTTTATCT CAGTGGCCTA CCCAAGAGGC
901      CGTGTGACTG AGGGGCTACA CCACCAGTAC TCAGAGAATG ATGAGAACTA CGTGGAAGTG
961      TCGCTGATTT TTGATCCAGT CACAAAGGAG GATCTGAATA CAGATTTTAA ATGTGTTGCC
1021     ACGAATCCAC GGAGTTTTCA GTCACTCCAT ACCACAGTCA AAGAAGTTTC TTCCACGTTC
1081     TCCTGGGGCA TCGCGCTGGC ACCTCTGTCT CTGATCATCT TGGTTGTGGG GGCGATATGG
1141     ATTCGCAGAA GGTGTAAACG CCAGGCTGGA AAGACGTATG GGCTGACCAA GCTACCGACT
1201     GACAACCAGG ACTTCCCTTC CAGCCCAAAC CAAATAAAGG AAATGAAATA A

 

Quality Control:cDNA sequencing results is available upon request.