Mouse IL1R2 / CD121b Protein (Cytokine)

Interleukin 1 receptor, type II Protein Datasheet

IL1R2 / CD121b Protein Product Information

• Synonym: IL1R2, Il-1r2, Il1rb
• Protein Construction: A DNA sequence encoding the mouse IL1R2 (P27931) (Met1- Glu355) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

IL1R2 / CD121b Protein QC Testing

• Purity: > 85% as determined by SDS-PAGE
• Bio-activity: Measured by its ability to bind biotinylated recombinant human IL1α (Cat:10128-HNCH) in a functional ELISA.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Phe 14
• Molecular Mass: The recombinant mouse IL1R2 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 583 amino acids and has a predicted molecular mass of 65.1 kDa. The apparent molecular mass of the protein is approximately 91 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL1R2 / CD121b protein

IL1R2 / CD121b Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL1R2 / CD121b Protein Description

The pleiotropic cytokine IL1 is produced to regulate development and maintenance of the inflammatory responses, and binds to specific plasma membrane receptors on cells. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified, designated as IL1RI (IL1RA) and IL1RII (IL1RB). A closely related receptor-like chain IL1R accessory protein (IL1RAcP) has been reported to heterodimerize with the IL1RI to form the signal transduction complex which appears to mediate all the known IL1 signal processes. IL1RII structurally consisting of a ligand binding portion comprised of three Ig -like domains, a single transmembrane region, and a short cytoplasmic domain, is expressed in a variety of cell types including B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Like the IL1RI, the human IL1RII can bind all three forms of IL1 (IL1 alpha, IL1 beta and IL1ra) but does not transmit IL1 signals. In addition to the membrane-bound form, IL1RII also exists as the soluble form, and either acts as a decoy receptor that inhibits IL1 action by blocking the binding of IL1 to the type I receptor complex. Furthermore, IL4 is reported to antagonize the activity of IL1 by inducing the expression and release of this cytokine. Recombinant soluble IL1RII is thus a potent antagonist of IL1 action, and serves as a potential therapeutic target.

References

1. McMahan CJ. et al., 1991, EMBO J. 10: 2821-32.
2. Liu C. et al., 1996, J Biol Chem. 271: 20965-72.
3. Rauschmayr T. et al., 1997, Proc Natl Acad Sci. 94: 5814-19.
4. Neumann D. et al., 2000, J Immunol. 165: 3350-7.
5. Colotta F. et al., 1993, Science. 261: 472-5.