Mouse IL1R1 / CD121a Protein (Cytokine)

Interleukin 1 receptor, type I Protein Datasheet

IL1R1 / CD121a Protein Product Information

• Synonym: CD121a, CD121b, IL-iR, Il1r-1, MGC129154
• Protein Construction: A DNA sequence encoding the mouse IL1R1 (P13504) extracellular domain (Met 1-Lys 338) was fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Mouse
• Expression Host: Human Cells

IL1R1 / CD121a Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.
  2. Immobilized recombinant human IL1b (Cat:10139-HNAE) at 10 μg/mL (100 μl/well) can mouse IL1R1-Fc (Cat:50807-M02H) with a linear range of 62.5-1000 ng/mL.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 20
• Molecular Mass: The secreted recombinant mouse IL1R1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 560 amino acids and has a calculated molecular mass of 64.3 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 70 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL1R1 / CD121a protein

IL1R1 / CD121a Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL1R1 / CD121a Protein Description

Interleukin 1 receptor, type I (IL1R1), also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor that belongs to the IL-1 receptor family. IL1R1 is a receptor for both interleukin alpha (IL1A) and interleukin beta (IL1B), and also associates with interleukin-1 receptor antagonist protein (IL-1RA). There are two distinct types of receptors that bind the pleiotropic cytokines IL1A and IL1B: the type I is an 80 kDa transmembrane protein expressed predominantly by T cells, fibroblasts, and endothelial cells and appears to mediate all the known IL-1 biological functions. However, the type a… receptor is a 68 kDa transmembrane protein with a short cytoplamic domain and serves as a decoy for IL-1. IL1R1 structurally consists of three C2-type I g-like domains and one TIR domain. As important mediators involved in many immune and inflammatory responses, the agonists IL1A and IL1B bind to IL1R1, leading to the signal transduction and activation of NF-kappa-B. Formation of a ternary complex containing IL1RAP, TOLLIP, MYD88, and IRAK1 or IRAK2 is indepensible for the signaling processes. Recombinant soluble form of IL1R1 is thus a potent antagonist of IL-1 function.

References

1. Reddy, S. A. et al., 1997, J. Biol. Chem. 272 : 29167-29173.
2. Huang, J. et al.,1997, Proc. Natl. Acad. Sci. U.S.A. 94: 12829-12832.
3. Vigers, G. P.A. et al., 1997, Nature. 386:190-194.
4. Schreuder, H. et al., 1997, Nature. 386:194-200.
5. Vigers, G.P.A. et al., 2000, J. Biol. Chem. 275: 36927-36933.