Mouse IL1F8 / IL36b Protein (Native)

Interleukin 1 family, member 8 Protein Datasheet

IL1F8 / IL36b Protein Product Information

• Synonym: RP23-176J12.1, 2310043N20Rik, Il36b
• Protein Construction: A DNA sequence encoding the mouse IL1F8 (Q9D6Z6) (Met 1-Lys 183 ) was expressed.
• Source: Mouse
• Expression Host: E.coli

IL1F8 / IL36b Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant mouse IL1F8 consisting of 183 amino acids and has a calculated molecular mass of 21 kDa as estimated in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 50mM Tris, pH8.0
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL1F8 / IL36b protein

IL1F8 / IL36b Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL1F8 / IL36b Protein Description

Interleukin-1 family member 8(IL1F8) also known as IL36B, is a member of the interleukin 1(IL-1) cytokine family. IL1F8 may contains a 12-stranded beta-trefoil structure. Until recently, the IL-1 family of cytokines includ four members, with three having pro-inflammatory effects such as IL1F8 and the fourth member being an IL-1 receptor antagonist (IL-1Ra). IL-1 family members exert their effects through binding to receptors that belong to the IL-1 receptor (IL-1R) family. IL1F8 exerts proinflammatory effects in primary human joint cells. Joint and serum IL-1F8 protein levels did not correlate with inflammation, but they were high in some human serum samples tested, including samples from patients with rheumatoid arthritis. IL1F8 also activated NK-κB.

References

1. Magne D. et al., 2006, Arthritis Research & Therapy. 8: R80.
2. Smith DE. et al., 2000, The Journal of Biological Chemistry. 275: 1169-75.