IL1F6 / IL36A Protein (His Tag) (Cytokine)

Interleukin 36, alpha Protein Datasheet

IL1F6 / IL36A Protein Product Information

• Synonym: FIL, 1E, IL1E, IL1F6, IL36A
• Protein Construction: A DNA sequence encoding the mature form of human IL1F6 (Q9UHA7) (Lys 6- Phe158) was expressed with a polyhistide tag at the N-terminus.
• Source: Human
• Expression Host: E.coli

IL1F6 / IL36A Protein QC Testing

• Purity: > 99 % as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human IL1F6 consists of 169 amino acids and predicts a molecular mass of 19.2 KDa. It migrates as an approximately 20 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL1F6 / IL36A protein

IL1F6 / IL36A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL1F6 / IL36A Protein Description

Interleukin-1 family member 6, also known as IL-1F6, FIL1 epsilon, Interleukin-1 epsilon, IL-1 epsilon, IL1F6 and FIL1E, is a secreted protein which belongs to the IL-1 family. IL-1F6 is a new IL-1 family member. IL1F6 / FIL1E is expressed in immune system, fetal brain and adipose tissue and that IL-1F6 and IL-1F8 are involved in the regulation of adipose tissue gene expression. Importantly, IL-1F6 inhibits PPARγ expression which may lead to reduced adipocyte differentiation suggesting metabolic effects of this cytokine.. IL1F6 / FIL1E, along with IL-36β / IL-1F8 and IL-36γ / IL-1F9, has been shown to act as an agonist by activating the pathway involving NF-κB and MAPK in an IL-1 Rrp2 dependent manner. IL1F6 / FIL1E may signal in a similar fashion to IL-1 and IL-18 in having a binding receptor which upon ligation, recruits a second receptor as a signaling component, forming an active heterodimeric receptor complex.

References

1. Smith DE. et al., 2000, J Biol Chem. 275: 1169-1175.
2. Dunn E. et al. 2001, Trends Immunol. 22-53.
3. Nicklin MJ. et al., 2002, Genomics. 79 (5): 718-25.
4. Blumberg H. et al., 2007, J Exp Med. 204 (11): 2603-14.
5. Dinarello C. et al., 2010, Nat Immunol. 11: 973.