Canine IL1B Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
DG70018-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL1B cDNA Clone Datasheet

IL1B cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001037971.1
cDNA Size:	798
Gene Synonym:	IL-1；IL1B
Source:	Canine

IL1B cDNA Clone Description

cDNA Description:	ORF Clone of Canis lupus familiaris interleukin 1, beta DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

IL1B cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL1B cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL1B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCAGCAG TACCCGAACT CACCAGTGAA ATGATGGCTT ACTCCAGTAA CAATGAGAAT
61       GACCTATTCT TTGAAGCTGA TGGCCCTGGA AATGTGAAGT GCTGCTGCCA AGACCTGAAC
121      CACAGTTCTC TGGTAGATGA GGGCATCCAG TTGCAAGTCT CCCACCAGCT CTGTAACAAG
181      AGTCTGAGGC ATTTCGTGTC AGTCATTGTA GCTTTGGAGA AGCTGAAGAA GCCCTGCCCA
241      CAGGTCCTCC AGGAGGATGA CCTGAAGAGC ATCTTTTGCT ACATCTTTGA AGAAGAACCT
301      ATCATCTGCA AAACAGATGC GGATAATTTT ATGAGTGATG CAGCCATGCA ATCGGTGGAC
361      TGCAAGTTAC AGGACATAAG CCACAAATAC CTGGTGCTGT CTAACTCATA TGAGCTTCGG
421      GCTCTCCACC TCAATGGGGA AAATGTGAAC AAACAAGTGG TGTTCCACAT GAGCTTTGTG
481      CACGGGGATG AAAGTAATAA CAAGATACCT GTGGTCTTGG GCATCAAACA AAAGAATCTG
541      TACCTGTCCT GTGTGATGAA GGATGGAAAG CCCACCCTAC AGCTAGAGAA GGTAGACCCC
601      AAAGTCTACC CAAAGAGGAA GATGGAAAAG CGATTTGTCT TCAACAAGAT AGAAATCAAG
661      AACACAGTGG AATTTGAGTC TTCTCAGTAC CCTAACTGGT ACATCAGCAC CTCTCAAGTC
721      GAAGGAATGC CTGTCTTCCT AGGAAATACC AGAGGTGGCC AGGATATAAC TGACTTCACC
781      ATGGAATTCT CTTCCTAG

Quality Control:cDNA sequencing results is available upon request.

Cytokine