Canine IL-1 beta / IL1B Protein (Native)

Interleukin 1, beta Protein Datasheet

IL-1 beta / IL1B Protein Product Information

• Synonym: IL-1
• Protein Construction: A DNA sequence encoding the mature form of canine IL1B (Q28292?) (Ala 115-Ser 266 ) was expressed, with an initial Met at the N-terminus.
• Source: Canine
• Expression Host: E.coli

IL-1 beta / IL1B Protein QC Testing

• Purity: > 96 % as determined by SDS-PAGE.
• Bio-activity: Measured by its ability to bind human IL1R1 in a functional ELISA.
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant canine IL1B consists of 153 amino acids and has a calculated molecular mass of 17.6 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 17KDa band.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM PB, 0.3M Nacl, pH6.5
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL-1 beta / IL1B protein

IL-1 beta / IL1B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL1B / IL-1beta Protein Description

Interleukin 1 (IL-1) is a family of polypeptide cytokines consisting of two agonists, IL-1alpha (IL-1F1) and IL-1beta (IL-1F2) encoded by two distinct genes and perform identical biological functions . IL1α and IL1β binds directly to a high-affinity receptor complex of IL1 RI and IL1 R AcP to initiate the signal transduction. IL1RII also binds to IL1β with high affinity, but on contrast, IL1β activity is inhibited since IL1RII exists as a decoy receptor and negative regulator.IL1R antgonist (IL1RA), also a member of IL-1 family, is identified to interact with IL1R1, preventing IL1α and IL1β to exert actions. IL1β is produced by a variety of cell types, and capable of stimulating thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. Also identified as endogenous pyrogens, IL-1 are involved in the immune and inflammatory responses, and are reported to stimulate the release of prostaglandin and collagenase. IL1β is encoded as a inactive precusor of 269 amino acids with a 116 aa propeptide which is cleaved intracellularly by the cysteine protease, IL1β converting enzyme (Caspase1/ICE) to generate the biologically active cytokine. Lacking of a specific hydrophobic segment suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

References

1. March C J. et al., 1985 , Nature. 315: 641-7.
2. Bensi G. et al., 1987, Gene. 52: 95-101.
3. Mizutani H. et al., 1991, J Exp Med. 174: 821-5.
4. Clore G M. et al., 1991, Biochemistry. 30: 2315-23.
5. Vigers G P A. et al., 1997, Nature. 386: 190-4.