Cynomolgus monkey IL1A Gene cDNA Clone / ORF Clone (Cytokine)

IL1A cDNA Clone Datasheet

IL1A cDNA Clone Product Information

• cDNA Size: 816
• Gene Synonym: IL1A
• Source: Cynomolgus monkey

IL1A cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) interleukin 1, alpha DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 30 C/A, 451 C/T 631-632 AA/CC resulting in the amino acid Asp substitution by Glu，His substitution by Tyr，Asn substitution by Pro and 831 A/C，975 T/C not causing the amino acid variation.
  Please check the sequence information before order.

IL1A cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL1A cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL1A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCAAAG TTCCAGACAT GTTTGAAGAA CTGAAGAACT GTTACAGTGA AAATGAAGAA
61       GACAGTTCCT CCATTGACCA TCTGTCTCTG AATCAGAAAT CCTTCTATGA TGTAAGCTAT
121      GGCCCACTCC ATGAAGGCTG CATGGATCAG TCTGTGTCTC TGAGTATCTC TGAAATCTCT
181      AAAACATCCA AGCTGACCTT CAAGCAGAGC ATGGTGGTAG TATCAACCAA TGGGAAGGTT
241      CTGAAGAAGA GACGGTTGAG TTTAAGCCAG TCCATCACTG ATAATAACCT GGAGGCCATC
301      GCCAACGACT CAGAGGAAGA AATCATCAAG CCCAGGTCGG CACCTTTTAG CTTCCTAAGC
361      AATATGACAT ACCACTTTAT AAGGATCATC AAACACGAAT TCATCCTGAA TGACACCCTC
421      AATCAAACTA TAATTCGAGC CAATGATCAG TACCTCACGG CTGCTGCAAT ACATAATCTG
481      GATGAAGCAG TGAAATTTGA CATGGGTGCT TATACGTCAT CAAAGGATGA TACTAAAGTT
541      CCTGTGATTC TAAGAATCTC AAAAACTCAA TTGTATGTGA GTGCCCAAGA TGAAGACCAA
601      CCGGTGCTGC TGAAGGAGAT GCCTGAGATA CCCAAAACCA TCACAGGTAG TGAGACCAAC
661      TTCCTCTTCT TCTGGGAAAC TCATGGCACT AAGAACTACT TCATATCAGT TGCCCATCCA
721      AACTTGTTTA TTGCCACAAA GCATGACAAT TGGGTGTGCT TGGCAAAGGG GCTACCCTCT
781      ATCACTGACT TTCAGATACT GGAAAACCAG GCGTAG

 

Quality Control:cDNA sequencing results is available upon request.