Rat IL1A Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80385-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL1A cDNA Clone Datasheet

IL1A cDNA Clone Product Information

Gene Bank Ref.ID:	NM_017019.1
cDNA Size:	813
Gene Synonym:	IL-1 alpha; Il1a
Source:	Rat

IL1A cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus interleukin 1 alpha DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

IL1A cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL1A cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL1A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCAAAG TTCCTGACTT GTTTGAAGAC CTAAAGAACT GCTATAGTGA AAATGAAGAA
61       TACAGTTCTG CCATTGACCA TCTGTCTCTG AATCAGAAAT CCTTCTATGA TGCAAGCTAT
121      GGCTCACTTC ATGAGAACTG CACAGATAAA TTTGTATCTC TGAGAACCTC TGAAACATCA
181      AAGATGTCCA CCTTCACCTT CAAGGAGAGC CGGGTGGTGG TGTCAGCAAC ATCAAACAAA
241      GGGAAGATTC TGAAGAAGAG ACGGCTAAGT TTCAATCAGC CCTTTACTGA AGATGACCTG
301      GAGGCCATAG CCCATGATTT AGAAGAGACC ATCCAACCCA GATCAGCACC TCACAGCTTC
361      CAGAATAATT TGAGATACAA ATTGATAAGG ATCGTCAAGC AGGAGTTCAT CATGAATGAT
421      TCCCTCAACC AAAATATATA TGTGGATATG GACAGAATAC ATCTCAAAGC TGCTTCGTTA
481      AATGACCTGC AGCTTGAAGT AAAATTTGAC ATGTATGCCT ACTCATCGGG AGGAGACGAC
541      TCTAAATATC CTGTGACTCT CAAAGTCTCA AATACTCAGC TCTTTGTGAG TGCTCAGGGA
601      GAAGACAAGC CTGTGTTGCT GAAGGAGATT CCGGAAACAC CAAAACTCAT CACAGGTAGT
661      GAGACCGACC TCATTTTCTT CTGGGAAAAA ATCAACTCTA AGAACTACTT CACATCCGCA
721      GCTTTCCCAG AGCTGTTAAT TGCCACAAAA GAACAAAGTC AGGTGCACCT GGCACGGGGA
781      CTGCCCTCCA TGATAGATTT CCAGATATCA TAA

Quality Control:cDNA sequencing results is available upon request.

IL1A / IL 1 alpha related areas, pathways, and other information

Cytokine