Canine IL1A Gene cDNA Clone / ORF Clone (Cytokine)
|Catalog||Vector (Price)||Quantity||In Stock||Operation|
IL1A cDNA Clone Datasheet
IL1A cDNA Clone Product Information
|Gene Bank Ref.ID:||NM_001003157.2|
IL1A cDNA Clone Description
ORF Clone of Canis lupus familiaris interleukin 1, alpha DNA.
|Vector:||Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
|Sequence Description:||Identical with the Gene Bank Ref.ID sequence.|
IL1A cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
IL1A cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
IL1A cDNA Clone Sequence and Quality Control
1 ATGGCCAAAG TTCCTGACCT CTTTGAAGAC CTGAAGAACT GTTACAGTGA AAATGAAGAA 61 TACAGTTCTG AAATTGACCA TCTCTCTCTG AATCAGAAAT CCTTCTATGA TATGAGCTGT 121 GACCCACTTC ATGAGGACTG CATGTCTCTG AGTACCTCTG AAATCTCAAA GACATCCCAG 181 CTTACCTTCA AGGAAAATGT GGTAGTGGTG GCAGCCAATG GGAAGATTCT AAAGAAGAGA 241 CGGTTGAGTT TAAGTCAATT CATCACCGAT GATGACCTGG AAGACATTGC CAATGACACA 301 GAAGAAGTAA TCATGAAGCC CAGATCAGTA GCATACAACT TCCATAACAA TGAAAAATAC 361 AACTATATAA GGATCATCAA ATCCCAATTC ATCCTGAATG ACAACCTCAA TCAAAGTATA 421 GTTCGACAAA CAGGAGGAAA TTACCTCATG ACTGCTGCAT TACAGAATTT GGATGATGCA 481 GTGAAGTTTG ACATGGGAGC TTACACATCA GAAGATTCTA AACTTCCTGT GACTCTAAGA 541 ATATCAAAAA CTCGACTCTT TGTGAGTGCC CAAAATGAAG ATGAACCTGT ATTGCTAAAG 601 GAGATGCCTG AGACACCCAA AACTATCAGA GATGAGACAA ACCTTCTTTT CTTTTGGGAG 661 CGTCATGGCA GTAAGCACTA CTTCAAATCA GTTGCCCAGC CCAAGTTGTT CATTGCCACA 721 CAGGAACGAA AACTGGTGCA CATGGCAAGA GGGCAACCCT CTATCACTGA CTTTCGGTTA 781 CTGGAAACCC AGCCTTGA
Quality Control:cDNA sequencing results is available upon request.