Canine IL1A Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
DG70017-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL1A cDNA Clone Datasheet

IL1A cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001003157.2
cDNA Size:	798
Gene Synonym:	IL1A
Source:	Canine

IL1A cDNA Clone Description

cDNA Description:	ORF Clone of Canis lupus familiaris interleukin 1, alpha DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

IL1A cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL1A cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL1A cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCCAAAG TTCCTGACCT CTTTGAAGAC CTGAAGAACT GTTACAGTGA AAATGAAGAA
61       TACAGTTCTG AAATTGACCA TCTCTCTCTG AATCAGAAAT CCTTCTATGA TATGAGCTGT
121      GACCCACTTC ATGAGGACTG CATGTCTCTG AGTACCTCTG AAATCTCAAA GACATCCCAG
181      CTTACCTTCA AGGAAAATGT GGTAGTGGTG GCAGCCAATG GGAAGATTCT AAAGAAGAGA
241      CGGTTGAGTT TAAGTCAATT CATCACCGAT GATGACCTGG AAGACATTGC CAATGACACA
301      GAAGAAGTAA TCATGAAGCC CAGATCAGTA GCATACAACT TCCATAACAA TGAAAAATAC
361      AACTATATAA GGATCATCAA ATCCCAATTC ATCCTGAATG ACAACCTCAA TCAAAGTATA
421      GTTCGACAAA CAGGAGGAAA TTACCTCATG ACTGCTGCAT TACAGAATTT GGATGATGCA
481      GTGAAGTTTG ACATGGGAGC TTACACATCA GAAGATTCTA AACTTCCTGT GACTCTAAGA
541      ATATCAAAAA CTCGACTCTT TGTGAGTGCC CAAAATGAAG ATGAACCTGT ATTGCTAAAG
601      GAGATGCCTG AGACACCCAA AACTATCAGA GATGAGACAA ACCTTCTTTT CTTTTGGGAG
661      CGTCATGGCA GTAAGCACTA CTTCAAATCA GTTGCCCAGC CCAAGTTGTT CATTGCCACA
721      CAGGAACGAA AACTGGTGCA CATGGCAAGA GGGCAACCCT CTATCACTGA CTTTCGGTTA
781      CTGGAAACCC AGCCTTGA

Quality Control:cDNA sequencing results is available upon request.

IL1A / IL 1 alpha related areas, pathways, and other information

Cytokine