Cynomolgus IL18RAP Protein (Cytokine)

Interleukin 18 receptor accessory protein Protein Datasheet

IL18RAP Protein Product Information

• Synonym: IL18RAP
• Protein Construction: A DNA sequence encoding the cynomolgus IL18RAP (F7HHC1) (Met1-Arg356) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Cynomolgus
• Expression Host: Human Cells

IL18RAP Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Phe 20
• Molecular Mass: The recombinant cynomolgus IL18RAP is a disulfide-linked homodimer. The reduced monomer comprises 578 amino acids and has a calculated molecular mass of 65.4 KDa.The apparent molecular mass of the protein is approximately 92 KDa respectively in SDS-PAGE.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL18RAP protein

IL18RAP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL18RAP Protein Description

Interleukin 18 receptor accessory protein, also known as IL18RAP, IL1R7 and CDw218b, is a single-pass type I  membrane protein. It is member of the interleukin-1 receptor family. IL18RAP contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 TIR domain. IL18RAP is strongly expressed in peripheral blood leukocytes, spleen, lung, and, to a lesser extent, in colon. It is not expressed in any other tissue tested. IL18RAP is also strongly expressed in T-cells polarized with IL12 and specifically coexpressed with IL18R1 in Th1 cells. IL18RAP is an accessory subunit of the heterodimeric receptor for IL18. This protein enhances the IL18 binding activity of IL18R1, the ligand binding subunit of IL18 receptor. The coexpression of IL18R1 and IL18RAP is required for the activation of NF-κB and MAPK8 (JNK) in response to IL18. Variants at IL18RAP have been linked to susceptibility to coeliac disease.

References

1. Born TL. et al., 1998, J Biol Chem. 273 (45): 29445-50.
2. Hillier LW. et al., 2005, Nature. 434: 724-31.
3. Hunt KA. et al., 2008, Nat Genet. 40 (4): 395-402.
4. Imami K. et al., 2008, Anal Sci. 24: 161-6.