Rat IL18R1 Gene cDNA Clone / ORF Clone

IL18R1 cDNA Clone Datasheet

IL18R1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001106905.2
• cDNA Size: 1614
• Gene Synonym: Il18r1, Il18r1_predicted
• Source: Rat

IL18R1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 18 receptor 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 1515 A/C resulting in the amino acid lys substitution by asn and 1263 C/T,1344 C/A not causing the amino acid variation.

IL18R1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL18R1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL18R1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCATCATG AAGAATTAAT CTTGATACTT TGTGTTCTCG TTGTTAAAAG TGCCCCGAAA
61       AGTTGCATTC GCCGGTCGCA AATTCACGTA GTAGAGGGAG AGCCTTTTTA TCTGAAGCCC
121      TGTGATATGT CTGCACCCAT GCATAACAAT GAGACAGCCA CCATGAGATG GTTCAAAGGC
181      AATGCTTCAC ATGGCTACAG AGAGCTGAAC ATGAGAAGCT CGCCCAGAAT CGCTTTTCAT
241      GGTCACGCTT TGGAATTCTG GCCAGTTGAG CTGGAGGACA AGGGAACGTA CTTTTCTCAA
301      GTGGGAAATG ATCGTCAAAA TTGGACCTTA AATGTCACCA AAAGAAACAA ACACAGCTGC
361      TTCTCTGAGA AGCTTGTGAC GAACAGAGAT GTGGAGGTTA AGAAATCTCT GTGGATTACC
421      TGTGAAAATC CTAGCTATGG AGAGCTGATC AACCACACGT TGCTGTATAA GAACTGTAAG
481      GAAATATCCA AAACCCCAAT GATCCTGAAG GATGCCGAGT TTGGAGATGA AGGTTATTAC
541      TCCTGCGTGT TTTCTGTCCA CCATAACGGG CAACAGTACA ACATCACCAA GACTGTCAAT
601      ATAACGGTTA TTGAAGGGAA CAGTAAAATA ACTCCGGCTA TTTTTGGATC AAAGTCTGCG
661      AAGGTTGGTG TAGAACTAGG AGAAGATGTG GAGCTGAACT GCAGTGCTGT GCTGAATAGA
721      AATGATCTGT TTTATTGGAG CATCAGGAAA GAGGACAGCT TAGACCCTAA CGTGCATGAA
781      GACAGGAACG AGACAACATG GACTTTTGAA GGCAAATTGC ATGCTTCAAA AATCCTGAGA
841      ATTCAGAAAG TTACAGAAAA ATATCTCAAT GTTTTATATA ATTGTACCGT GGCCAACGAA
901      GAAGCCACAG ACACCAAAAG CTTCATCTTG GTGAGAAAAG AAACACCTGA CATCCAAGGC
961      CATGTCTTTA TGAGAGGAAT AACCATGGTT GTTCTCACCT CTGTGGCAGC AGTGTGCGTG
1021     GTGATTTTGT GCATCATTTA TAAAGTTGAT TTGGTTTTAT TCTATAGACG TGTAGCAGAA
1081     AGAGACGAGA CACTAACAGA TGGTAAAACA TATGATGCCT TTGTGTCTTA CCTGAGAGAG
1141     TGTCATCCTG AGAATGGAGA AGAGTACACT TTTGCTGTGG AGACGTTACC CAGGGTCCTG
1201     GAGAAACAGT TTGGCTATAA GTTGTGCATA TTTGAAAGAG ATGTGGTGCC TGGTGGAGCT
1261     GTTGTTGATG AGATCCATTC ACTGATAGAG AAAAGCCGGA GGCTAATCAT CGTCCTCAGC
1321     AAGAGTTACA TGACTAATGG ATCAAGGCTC GAGCTGGAGA GCGGACTCCA CCAAGCACTG
1381     GTGGAGAGGA AGATTAAAAT AATCTTAATC GAGTTTACTC CAGCCAGCAA CATCACCTTT
1441     CTCCCGCCGT CGCTGAAACT CCTCAAGTCC TACAGAGTTC TGAGGTGGAA GGCTGACAAG
1501     CCACTCTCTT CGAACTCACG ATTCTGGAAG AATCTTCTTT ACCTGATGCC TGCCAAAGCC
1561     GTCAAGCCAT GGAGAGAAGA ATCAGAGGCC CGGCCTGTTC TCTCAGCACC TTGA

 

Quality Control:cDNA sequencing results is available upon request.