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IL18R1 ELISA Pair Set, Mouse ELISA Pair Set (Cytokine) 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK50094 |
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Mouse IL18R1 ELISA Pair Set
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Order or Inquire for IL18R1 ELISA Pair Set |
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High affinity Mouse IL18R1 ELISA Pair Set |
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Detection limit - 15.625 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Mouse IL18R1 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
The interleukin-18 receptor 1 (IL18R1), also known as CDw218a (cluster of differentiation w218a), is a cytokine receptor that belongs to the interleukin 1 receptor family, and a single-pass type I membrane protein contining three Ig-ike C2-type domains within the ECD. It specifically binds interleukin 18 (IL18) and is essential for IL18 mediated signal transduction, and it is reported that binding to the agonist leads to the activation of NF-kappa-B. IL-18 receptor was first described in 1989. It induces synthesis and release of IFN-gamma, IL- 8, 4 and 13, TNF-alpha and granulocyte/macrophage colony stimulating factor (GM-CSF). It modulates the function of many immunocompetent cells including macrophages, monocytes, lymphocytes and granulocytes. Another feature of IL-18 receptor is its ability to induce apoptosis of many cell types by inducing Fas ligand and receptor, aas well as granzymes. High level of IL18R1 expression is detected in Hodgkin's disease cell lines.
