Canine IL18 Gene cDNA Clone / ORF Clone (Cytokine)
|Catalog||Vector (Price)||Quantity||In Stock||Operation|
IL18 cDNA Clone Datasheet
IL18 cDNA Clone Product Information
|Gene Bank Ref.ID:||NM_001003169.1|
IL18 cDNA Clone Description
ORF Clone of Canis lupus familiaris interleukin 18 (interferon-gamma-inducing factor) DNA.
|Vector:||Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
|Sequence Description:||Identical with the Gene Bank Ref. ID sequence except for the point mutation 36 C/T not causing the amino acid variation.|
IL18 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
IL18 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
IL18 cDNA Clone Sequence and Quality Control
1 ATGGCTGCTA ACCTAATAGA AGACAATTGC ATCAATCTTG TGAAAATGAA ATTTGTTAAC 61 AATACACTGT ACTTTAAAGC GGAAAGTGAT GAAGGCCTGG AATCAGATTA CTTTGGCAAG 121 CTTGAACCTA AACTCTCAAT CATACGAAAT TTGAACGACC AAGTCCTCTT CGTTAACGAG 181 GGAAATCAAC CTGTATTTGA GGATATGCCC GATTCTGACT GTACAGATAA TGCACCCCAT 241 ACCATATTTA TCATCTATAT GTATAAAGAT AGCCTCACTA GAGGTCTGGC AGTAACTATC 301 TCTGTGAAGT ATAAGACAAT GTCTACTCTC TCCTGTAAGA ACAAAACTAT TTCCTTTCAG 361 AAAATGAGTC CTCCGGATAG TATCAATGAT GAAGGAAATG ACATCATATT CTTTCAGAGA 421 AGTGTTCCAG GCCATGATGA TAAGATACAA TTTGAGTCTT CATTGTACAA AGGACACTTT 481 CTAGCTTGTA AAAAAGAGAA CGATCTTTTC AAACTCATTT TGAAAGACAA GGATGAAAAT 541 GGGGATAAAT CCATAATGTT CACTGTTCAA AACAAGAGCT AG
Quality Control:cDNA sequencing results is available upon request.