Canine IL18 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
DG70020-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL18 cDNA Clone Datasheet

IL18 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001003169.1
cDNA Size:	582
Gene Synonym:	IGIF;IL18
Source:	Canine

IL18 cDNA Clone Description

cDNA Description:	ORF Clone of Canis lupus familiaris interleukin 18 (interferon-gamma-inducing factor) DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 36 C/T not causing the amino acid variation.

IL18 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL18 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL18 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCTGCTA ACCTAATAGA AGACAATTGC ATCAATCTTG TGAAAATGAA ATTTGTTAAC
61       AATACACTGT ACTTTAAAGC GGAAAGTGAT GAAGGCCTGG AATCAGATTA CTTTGGCAAG
121      CTTGAACCTA AACTCTCAAT CATACGAAAT TTGAACGACC AAGTCCTCTT CGTTAACGAG
181      GGAAATCAAC CTGTATTTGA GGATATGCCC GATTCTGACT GTACAGATAA TGCACCCCAT
241      ACCATATTTA TCATCTATAT GTATAAAGAT AGCCTCACTA GAGGTCTGGC AGTAACTATC
301      TCTGTGAAGT ATAAGACAAT GTCTACTCTC TCCTGTAAGA ACAAAACTAT TTCCTTTCAG
361      AAAATGAGTC CTCCGGATAG TATCAATGAT GAAGGAAATG ACATCATATT CTTTCAGAGA
421      AGTGTTCCAG GCCATGATGA TAAGATACAA TTTGAGTCTT CATTGTACAA AGGACACTTT
481      CTAGCTTGTA AAAAAGAGAA CGATCTTTTC AAACTCATTT TGAAAGACAA GGATGAAAAT
541      GGGGATAAAT CCATAATGTT CACTGTTCAA AACAAGAGCT AG

Quality Control:cDNA sequencing results is available upon request.

IL-18 related areas, pathways, and other information

Cytokine