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IL17RD ELISA Pair Set, Human ELISA Pair Set PDF Download

Catalog Size (Price) Quantity In Stock Operation Other Information
SEK10507

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Human IL17RD ELISA Pair Set

Order or Inquire for IL17RD ELISA Pair Set
High affinity Human IL17RD ELISA Pair Set
Detection limit - 78.125 pg/ml
Affordable price and 30%-80% cost saving for Bulk order
  • Materials
  • Protocol
  • Trouble Shooting
  • Image & Flash
     

Problems

Possible Sources

Solutions

No signal

Incorrect or no Detection Antibody was added

Add appropriate Detection Antibody and continue

Substrate solution was not added

Add substrate solution and continue

Incorrect storage condition

Check if the kit is stored at recommended condition and used before expiration date

     

Poor Standard Curve

Standard was incompletely reconstituted or was inappropriately stored

Aliquot reconstituted standard and store at  -70 ℃

Imprecise / inaccurate pipetting

Check / calibrate pipettes

Incubations done at inappropriate temperature, timing or agitation

Follow the general ELISA protocol

Background wells were contaminated

Avoid cross contamination by using the sealer appropriately

     

Poor detection value

The concentration of antigen in samples was too low

Enriching samples to increase the concentration of antigen

Samples were ineffective

Check if the samples are stored at cold environment. Detect samples in timely manner

     

High Background

Insufficient washes

Use multichannel pipettes without touching the reagents on the plate

Increase cycles of washes and soaking time between washes

TMB Substrate Solution was contaminated

TMB Substrate Solution should be clear and colorless prior to addition to wells

Materials were contaminated

Use clean plates, tubes and pipettes tips

     

Non-specificity

Samples were contaminated

Avoid cross contamination of samples

The concentration of samples was too high

Try higher dilution rate of samples

 

 

Human IL17RD ELISA Pair Set Related Products & Topics

Related Areas:

Immunology>>Adaptive Immunity>>T Cell>>Helper T Cell>>IL-17RD

Immunology>>Cytokine & Receptor>>Interleukin & Receptor>>IL-17 Family & IL-17 Receptor Family>>IL-17RD

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
IL-17RD Human IL-17RD Protein, Recombinant 10507-H08H

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human
IL-17RD
WB, ELISA IL-17RD Antibody, Rabbit Mab 10507-R010
Human
IL-17RD
ELISA Rabbit Polyclonal Antibody 10507-RP01
Human
IL-17RD
WB, ELISA Rabbit Polyclonal Antibody (Antigen Affinity Purified) 10507-RP02

Background

Human SEF (similar expression to fgf genes), also known as interleukin 17 receptor D (IL17 Rd), is a type I transmembrane receptor that shares sequence homology with the intracellular domain of members of the IL-17 receptor family. It is highly expressed in umbilical vein endothelial cells and in several highly vascularized tissues such as kidney, colon and heart. Two transcript variants resulting from alternative splicing exit, and the full-length long form is found in both the cytoplasm and plasma membrane. SEF is highly conserved in zebrafish, mouse, and humans, and studies in each system indicate that SEF acts as a feedback inhibitor of FGF-mediated Ras-MAPK signaling and ERK activation by inhibiting FGFR1 tyrosine phosphorylation and/or Ras downstream events. Additionally, SEF is also identified as a mediator of JNK and apoptosis by activating JNK through a TAK1-MKK4-JNK pathway. Human SEF is likely to play critical roles in endothelial or epithelial functions such as proliferation, migration, and angiogenesis.