Cynomolgus IL17RA / IL17R Protein (Cytokine)

Interleukin 17 receptor A Protein Datasheet

IL17RA / IL17R Protein Product Information

• Synonym: IL17RA
• Protein Construction: A DNA sequence encoding the cynomolgus IL17RA (Met1-Trp320) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Cynomolgus
• Expression Host: Human Cells

IL17RA / IL17R Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 33
• Molecular Mass: The recombinant cynomolgus IL17RA is a disulfide-linked homodimer. The reduced monomer comprises 529 amino acids and has a calculated molecular mass of 60.3 KDa.The apparent molecular mass of the protein is approximately 75-98 KDa respectively in SDS-PAGE.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL17RA / IL17R protein

IL17RA / IL17R Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL17RA / IL17R Protein Description

Interleukin-17 receptor A, also known as IL-17 receptor A, IL-17RA, IL17RA and IL17R, is an ubiquitous type I  membrane glycoprotein that binds with low affinity to interleukin 17A (IL17A). IL17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. IL17A binds to a type I  cell surface receptor called IL17R of which there are at least three variants IL17RA, IL17RB, and IL17RC. IL17RA is a widely expressed glycoprotein that plays a central role in inflammatory responses. IL17R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. The aa sequence of mouse IL17RA is 69% identical to the human IL17R. Within the extracellular domain, mouse IL17RA shares 72% aa sequence identity with human IL17RA. IL17RA associates with IL17RC to form a signaling receptor complex for IL17 and IL17F. Ligand and IL17RA ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple proinflammatory chemokines.

References

1. Yao Z. et al., 1997. Cytokine. 9 (11): 794-800.
2. Schwander R. et al., 2000, J Exp Med. 191:1233-40.
3. Starnes T. et al., 2002, J Immunol. 169 (2): 642-6.
4. Toy D. et al., 2006, J Immunol. 177 (1): 36-9.
5. Takahashi N. et al., 2008, J Exp Med. 205 (8): 1755-61.
6. Smith E. et al., 2008, J Immunol. 181 (2): 1357-64.