Mouse IL17F Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG51097-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL17F cDNA Clone Datasheet

IL17F cDNA Clone Product Information

Gene Bank Ref.ID:	NM_145856.2
cDNA Size:	486
Gene Synonym:	C87042; IL-17F
Source:	Mouse

IL17F cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus interleukin 17 F DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 462 G/A not causing the amino acid variation.

IL17F cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL17F cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL17F cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAGTGCA CCCGTGAAAC AGCCATGGTC AAGTCTTTGC TACTGTTGAT GTTGGGACTT
61       GCCATTCTGA GGGAGGTAGC AGCTCGGAAG AACCCCAAAG CAGGGGTTCC TGCCTTGCAG
121      AAGGCTGGGA ACTGTCCTCC CCTGGAGGAT AACACTGTGA GAGTTGACAT TCGAATCTTC
181      AACCAAAACC AGGGCATTTC TGTCCCACGT GAATTCCAGA ACCGCTCCAG TTCCCCATGG
241      GATTACAACA TCACTCGAGA CCCCCACCGG TTCCCCTCAG AGATCGCTGA GGCCCAGTGC
301      AGACACTCAG GCTGCATCAA TGCCCAGGGT CAGGAAGACA GCACCATGAA CTCCGTCGCC
361      ATTCAGCAAG AAATCCTGGT CCTTCGGAGG GAGCCCCAGG GCTGTTCTAA TTCCTTCAGG
421      TTGGAGAAGA TGCTCCTAAA AGTTGGCTGC ACCTGTGTCA AACCCATTGT CCACCAAGCG
481      GCCTGA

Quality Control:cDNA sequencing results is available upon request.

Cytokine