IL17 / IL17A Protein (His Tag) (Cytokine)

Interleukin 17A Protein Datasheet

IL17 / IL17A Protein Product Information

• Synonym: IL17A, CTLA8, IL17
• Protein Construction: A DNA sequence encoding the mature form of human IL17A(Q16552) (Gly24-Ala155) was expressed, with a polyhistidine tag at the N-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

IL17 / IL17A Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His
• Molecular Mass: The recombinant human human IL17A consists of 152 amino acids and predicts a molecular mass of 17.3 KDa. It migrates as an approximately 19 KDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL17 / IL17A protein

IL17 / IL17A Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL17 / IL17A Protein Description

IL17, also known as IL17a, is a member of the IL-17 family. The IL-17 family of cytokines includes six members, IL-17/IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F, which are produced by multiple cell types. Members of this family have a highly conserved C-terminus containing a cysteine-knot fold structure. Most IL-17 proteins are secreted as disulfide-linked dimers, with the exception of IL-17B, which is secreted as a non-covalent homodimer IL17 is a proinflammatory cytokine produced by activated T cells. It regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. IL17 also induces stromal cells to produce proinflammatory and hematopoietic cytokines. It enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts.

References

1. Andoh A. et al., 2002, J Immunol. 169 (4): 1683-7.
2. Kotake S. et al., 1999, J Clin Invest. 103 (9): 1345-52.
3. Laan M. et al., 1999, J Immunol. 162 (4): 2347-52.
4. Shin HC. et al., 1999, Cytokine. 10 (11): 841-50.