Rat IL15 Gene cDNA Clone / ORF Clone (Cytokine)

IL15 cDNA Clone Datasheet

IL15 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_013129.2
• cDNA Size: 489
• Gene Synonym: Il15
• Source: Rat

IL15 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 15 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

IL15 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL15 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL15 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAAAATTT TGAAACCATA TATGAGGAAT ACATCCATCT TGTACTACCT GTGTTTCCTT
61       CTCAACAGTC ACTTCTTAAC TGAGGCTGGC ATCCATGTCT TCATTTTGGG CTGTGTCAGT
121      GTAGGTCTCC CTAAAACAGA GGCCAACTGG ATAGATGTAA GATACGATCT GGAGAAAATT
181      GAAAGTCTTA TTCAATTTAT TCATATTGAT ACTACTCTAT ACACTGACAG TGACTTTCAT
241      CCCAGTTGCA AAGTTACTGC AATGAACTGC TTTCTCCTGG AATTACAGGT TATTTTGCAC
301      GAGTACAGTA ACATGACTCT TAATGAAACA GTAAGAAACG TGCTCTACCT TGCAAACAGC
361      ACTCTGTCTT CTAACAAGAA TGTAATAGAG TCTGGCTGCA AGGAATGTGA GGAGCTGGAG
421      GAGAGAAACT TCACGGAGTT TTTGCAGAGT TTTATACATA TTGTCCAAAT GTTCATCAAC
481      ACGTCCTGA

 

Quality Control:cDNA sequencing results is available upon request.