Rat IL13RA2 / IL13R Protein (Cytokine)

Interleukin 13 receptor, alpha 2 Protein Datasheet

IL13RA2 / IL13R Protein Product Information

• Synonym: IL13RA2
• Protein Construction: A DNA sequence encoding the rat IL13RA2 (Q8VHK6) (Met1-Lys336) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

IL13RA2 / IL13R Protein QC Testing

• Purity: > 90% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 24
• Molecular Mass: The recombinant rat IL13RA2/Fc is a disulfide-linked homodimer. The reduced monomer comprises 554 amino acids and has a predicted molecular mass of 63.4 kDa. The apparent molecular mass of the protein is approximately 89-93 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL13RA2 / IL13R protein

IL13RA2 / IL13R Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL13RA2 / IL13R Protein Description

Interleukin-13 receptor alpha2 chain(IL-13Rα2) is one of the receptor components for IL-13, a Th2 cell-derived pleiotropic immune regulatory cytokine. IL13Rα2 contains 3 fibronectin type-III domains, and binds as a monomer with high affinity to IL-13. IL-13Rα2 exists both on the cell membrane and in soluble form intracellularly which are generated from distinct transcripts. IL-13Rα2 was initially cloned from a kidney cell line and then found to be expressed in brain, spleen, liver, thymus, and also in the bone marrow, which are hemopoietic or hypervascular tissues. Soluble IL-13Rα2 was found in multiple mouse organs and was up-regulated in lungs following allergen challenge. IL-13Rα2 is expressed on certain types of cancer cells including glioblastoma, AIDS Kaposi's sarcoma, and head and neck cancer. In a IL13Rα2 transgenic mice model, the antitumor activity of IL13-PE, a receptor-directed cytotoxin was profoundly enhanced, providing elimination of established tumor in some xenografts. Comparisons of the human and murine receptors show that they are structurally conserved, share 59% aa identity.

References

1. Kawakami K. 2005, Curr Gene Ther. 5 (2): 213-23.
2. Tabata Y. et al., 2007, Curr Allergy Asthma Rep. 7 (5): 338-45.
3. Yasuhiro T. et al., 2006, The Journal of Immunology. 177: 7905-12.
4. Debra D Donaldson. et al., 1998, The Journal of Immunology. 161: 2317-24.