IL-13 / ALRH Antibody (Antigen Affinity Purified) (Cytokine)

IL13 / ALRH Antibody ( Antigen Affinity Purified ) Datasheet

IL13 / ALRH Antibody Product Information

• Immunogen: Recombinant Human IL13 protein (Catalog#10369-H01H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human IL13 / ALRH (rh IL13 / ALRH; Catalog#10369-H01H; AAK53823.1; Gly 21-Asn 132). IL13 / ALRH specific IgG was purified by Human IL13 / ALRH affinity chromatography.

IL13 / ALRH Antibody Usage Guide

• Specificity: Human IL13 / ALRH
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL13 in WB. Using a DAB detection system, the detection limit for Human IL13 is approximately 0.5 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL13 . The detection limit for Human IL13 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL13 / ALRH Antibody Background

Interleukin 13 (IL-13) is a single-chain cytokine which belongs to the IL13/IL4 family. In humans, IL13 is secreted by many cell types, but especially by T helper type 2 (Th2) cells. IL13 inhibits the production of pro-inflammatory cytokines and chemokines, and thus down-regulates macrophage activity. Meanwhile, IL-13 synergizes with IL2 in regulating IFN-gamma synthesis. IL-13 binds to a multi-subunit receptor comprising the alpha chain of the IL-4 receptor (IL-4Rα) and at least one of two known IL-13-specific binding chains (IL13 Rα1 and IL13 Rα2), and exerts its effects through signal transducer and activator of transcription 6 (STAT6), like IL-4. Accordingly, IL-13 may be critical in regulating inflammatory and immune responses as a central mediator in various cell types.

References

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