Rat IL12B Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80352-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL12B cDNA Clone Datasheet

IL12B cDNA Clone Product Information

Gene Bank Ref.ID:	NM_022611.1
cDNA Size:	1008
Gene Synonym:	Il12; Il12b
Source:	Rat

IL12B cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus interleukin 12b DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 505-506 CG/GC resulting in the amino acid Arg substitution by Ala.

IL12B cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL12B cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL12B cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGTCATC AGAAGTTAAC CTTCTCCTGG TTTGCCATGG TTTTGCTGGT GTCTCCACTC
61       ATGGCCATGT GGGAGCTGGA GAAAGATGTT TATGTTGTAG AGGTGGACTG GCGCCCCGAT
121      GCCCCTGGAG AAACGGTGAC CCTCACCTGT GACAGTCCTG AAGAAGATGA CATCACCTGG
181      ACCTCAGACC AGAGACGTGG AGTCATAGGC TCTGGAAAGA CCCTGACCAT CACTGTCAGA
241      GAGTTTCTAG ATGCTGGCCA ATACACCTGC CACAGAGGAG GCGAGACTCT GAGCCACTCA
301      CATCTGCTGC TCCACAAGAA GGAAAATGGA ATTTGGTCCA CCGAGATTTT AAAAAATTTC
361      AAAAATAAGA CTTTCCTGAA GTGTGAAGCA CCAAACTACT CCGGACGGTT CACCTGCTCA
421      TGGCTGGTGC ACAGAAACAC GGACTTGAAG TTTAACATCA AGAGCAGCAG CAGTTCCCCT
481      GAGTCTCGGG CGGTGACATG TGGAGCAGCA TCTCTGTCTG CAGAGAAGGT CACACTGAAC
541      CAAAGGGACT ACGAGAAGTA CTCAGTGGCG TGCCAGGAGG ACGTCACCTG CCCAACTGCC
601      GAGGAGACCC TGCCCATTGA ACTGGTGGTG GAGGCCCAGC AGCAGAATAA ATATGAGAAC
661      TACAGCACCA GCTTCTTCAT CAGGGACATC ATCAAACCGG ACCCACCCAA GAACCTGCAG
721      GTGAAACCTT TGAAGAACTC TCAGGTGGAG GTCAGCTGGG AGTACCCTGA CTCCTGGAGC
781      ACTCCCCATT CCTACTTCTC CCTCAAGTTC TTCGTCCGCA TCCAGCGCAA GAAAGAAAAG
841      ACGAAGGAGA CAGAGGAGGA GTGTAACCAG AAAGGTGCGT TCCTCGTAGA GAAGACCTCT
901      GCCGAAGTCC AATGCAAAGG GGCGAATATC TGCGTGCAAG CGCAGGACCG CTACTACAAT
961      TCATCATGCA GCAAATGGAC ATGTGTACCC TGCAGGGGCC GATCCTAA

Quality Control:cDNA sequencing results is available upon request.

IL12B/IL-12B related areas, pathways, and other information

Cytokine