Mouse IL12B / IL-12B Protein (His Tag) (Cytokine)

Interleukin 12b Protein Datasheet

IL12B / IL-12B Protein Product Information

• Synonym: IL12B
• Protein Construction: A DNA sequence encoding the mouse IL12B (Met1-Ser335) was expressed with a C-terminal polyhistidine tag.
• Source: Mouse
• Expression Host: Human Cells

IL12B / IL-12B Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 23
• Molecular Mass: The recombinant mouse IL12B comprises 324 amino acids and has a predicted molecular mass of 37.2 kDa. The apparent molecular mass of the protein is approximately 47-51 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL12B / IL-12B protein

IL12B / IL-12B Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL12B / IL-12B Protein Description

Interleukin 12 (IL-12), also known as NKSF or CLMF, is a disulfide-linked heterodimeric cytokine composed of a 35kDa subunit P35 and a 40kDa subunit P40, also designated as IL-12A and IL-12B. IL-12 is predominantly produced by macrophages and B lymphocytes and plays an important role in the activities of natural killer cells and T lymphocytes. It is involved in the differentiation and development of Th1 cells, enhancement of natural killer cells’ cytolytic function and mitogenic effects, as well as induction of IFN-gamma during which it can synergize with other IFN-gamma inducers. A large excess of monomeric IL-12B is also secreted by the cells producing IL-12, and exhibits no demonstrable biological activity. Overexpression of IL-12B has been shown to be associated with the pathogenesis of multiple sclerosis.

References

1. Ling, P. et al., 1995, J. Immunol. 154: 116-27.
2. Thierfelder, WE. et al., 1996, Nature. 382: 171-4.
3. Kato, K. et al., 1996, Proc. Natl. Acad. Sci. 93: 9085-89.
4. Kim, JH. et al., 1997, Clin. Exp. Immunol. 108: 243-50.