Anti-IL-12 p70 Antibody

Cat: 10021-RP02
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All IL-12 p70 Reagents
Anti-IL-12 p70 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-IL-12 p70 Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Human
Specificity
Human IL-12 p70
Immunogen
Recombinant Human IL12A protein (Catalog#10021-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human IL12A (rh IL12A; Catalog#10021-H08H; NP_000873.2; Met 1-Ser 219). IL12A IgG was purified by human IL12A affinity chromatography .
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IL-12 p70 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IL-12 p70 Antibody (Rabbit Polyclonal antibody) Images
Human IL12A / IL-12A Immunohistochemistry(IHC) 4016
Immunochemical staining of human IL12a in human breast carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human IL-12 p70 Western blot (WB) 19017

Anti-IL12A rabbit polyclonal antibody at 1:500 dilution

Lane A: MOLT4 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:26 kDa

Observed band size:38 kDa

(We are unsure as to the identity of these extra bands.)

Anti-IL-12 p70 Antibody Alternative Names
Anti-CLMF Antibody;Anti-IL-12A Antibody;Anti-NFSK Antibody;Anti-NKSF1 Antibody;Anti-P35 Antibody
IL-12 p70 Background Information

Interleukin-12 subunit alpha (IL12A/IL-12p35) is also known as Cytotoxic lymphocyte maturation factor 35 kDa subunit, cytotoxic lymphocyte maturation factor 1, p35, NK cell stimulatory factor chain 1, and interleukin-12 alpha chain. IL12A/IL-12p35 is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. IL12A/IL-12p35 is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. In clinical, IL-12 remains a very promising immunotherapeutic agent because recent cancer vaccination studies in animal models and humans have demonstrated its powerful adjuvant properties. The immune modulating characteristics of IL-12 considered responsible for the adjuvant effects, as well as the results of animal and human cancer vaccination studies with IL-12 applied as an adjuvant. IL12A/IL-12p35 indicates a cytokine which is important in the development of prostate cancer.

Full Name
interleukin 12A
References
  • Sattler HP, et al. (2000) Novel amplification unit at chromosome 3q25-q27 in human prostate cancer. Prostate. 45(3): 207-15.
  • Lamont AG, et al. (1996) IL-12: a key cytokine in immune regulation. Immunol Today. 17(5): 214-7.
  • Portielje JE, et al. (2003) IL-12: a promising adjuvant for cancer vaccination. Cancer Immunol Immunother. 52(3): 133-44.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IL-12 p70 Antibody Rabbit Polyclonal WB 100321-T32
    Anti-IL-12 p70 Antibody Rabbit Polyclonal ELISA 10021-RP01
    Anti-IL-12 p70 Antibody Mouse Monoclonal ELISA 10021-MM05
    Anti-IL-12 p70 Antibody Rabbit Monoclonal ELISA 10021-R006
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