IL11RA / IL11Rα Antibody (Cytokine)

Mouse Interleukin-11 Receptor Alpha Chain Antibody

IL11RA / IL11Rα Antibody Product Information

• Immunogen: Recombinant mouse IL11RA protein ( Catalog #50075-M08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 025
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant mouse IL11RA ( rM IL11RA ; Catalog #50075-M08H ; NP_001156873.1 ; Met 1 - Gln 367 )

IL11RA / IL11Rα Antibody Usage Guide

• Specificity: Mouse IL11RA / IL11Rα
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse IL11RA in WB. Using a DAB detection system, the detection limit for mouse IL11RA is approximately 0.25 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse IL11RA. The detection limit for mouse IL11RA is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL11RA / IL11Rα Antibody Background

Mouse interleukin-11 receptor alpha chain, also known as IL-11R-alpha, and IL11RA, is a single-pass type I  membrane protein. It belongs to the type I  cytokine receptor family and type 3 subfamily and contains two fibronectin type-III domains and one Ig-like C2-type (immunoglobulin-like) domain. Interleukin 11 receptor is a heterodimer composed of an interleukin 11 receptor alpha subunit and an incompletely characterized beta subunit. IL11RA is expressed in a number of cell lines, including the myelogenous leukemia cell line K562, the megakaryocytic leukemia cell line Mo7E, the erythroleukemia cell line TF1, and the osteosarcoma cell lines, MG-63 and Saos-2. It is also expressed in normal and malignant prostate epithelial cell lines. IL11RA is a receptor for interleukin-11. The receptor systems for IL6, LIF, OSM, CNTF, IL11 and CT1 can utilize IL6ST for initiating signal transmission. The IL11/IL11RA/IL6ST complex may be involved in the control of proliferation and differentiation of skeletogenic progenitor or other mesenchymal cells.

References

1. Chérel, M. et al., 1995, Blood. 86 (7): 2534-40. 
2. Chérel, M. et al., 1996, Genomics. 32 (1): 49-53.
3. Van Leuven, F. et al., 1996, Genomics 31 (1): 65-70.
4. Li, R. et al., 2001, Reproduction. 122 (4): 593-600.