|Datasheet||Specific References||Reviews||Related Products||Protocols|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Human IL11 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||HG12225-G-F|
|Human IL11 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG12225-G-H|
|Human IL11 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG12225-G-M|
|Human IL11 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG12225-G-N|
|Human IL11 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG12225-G-Y|
IL11 is a multifunctional cytokine first isolated in 1990 from bone marrow-derived stromal cells. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL11 is also known under the names adipogenesis inhibitory factor (AGIF) and oprelvekin. IL11 can improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. IL11 stimulates the growth of certain lymphocytes and, in the murine model, stimulates an increase in the cortical thickness and strength of long bones. As a signaling molecule, IL11 has a variety of functions associated with its receptor interleukin 11 receptor alpha; such functions include placentation and to some extent of decidualization.