Rat IL10Ra Gene cDNA Clone / ORF Clone

IL10Ra cDNA Clone Datasheet

IL10Ra cDNA Clone Product Information

• Gene Bank Ref.ID: NM_057193.2
• cDNA Size: 1710
• Gene Synonym: Il10ra, IL-10ra
• Source: Rat

IL10Ra cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus interleukin 10 receptor, alpha DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 501 C/T, 519 T/C and 1689 C/T not causing the amino acid variation.

IL10Ra cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL10Ra cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL10Ra cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTGCCGC GTTTACTCCC ATTCCTCGTC TCGATCTCCA GCCTGAGCCT GGGCTTCCGC
61       GCACACGGGA CAGAACTGCC GAGCCCTTCC TCCGTGTGGT TTGAAGCCAG ATTTTTCCAG
121      CACATCCTCC GCTGGATGTC TATCCCAAAC CAGTCTGAGA GCACCTACTA CGAAGTGGCC
181      CTCAAGAAGT ACGGAACCTC GGTCTGGAAA GACATCCACA TCTGTAGCAA GGCTCAGACA
241      TTGTCCTGCG ATCTCACAAC GTCCACCCTA GATCTGTACC ACAGCTCTGG CTACCGGGCC
301      AGAGTCCGGG CAGTGGACAA CAGTCAGTAT TCCAACTGGA CCATCACTGA AACTCGCTTC
361      ACAGTGGATG AAGTGATTCT GACGGTGGAT AGCGTGACGC TGAAAGTAAG CAACGGCTTC
421      ATCTACGGGA CAATCCATCC CCCCAGGCCC AATTTAGTCC CTGTAGGTGA CGAGTATGAA
481      CAGATCTTCA CGCACCACCG TATTTACAGG GTTTGCATCC GGAAGTTCTC AGAACAAAAG
541      AATATAACCA AGACAGTGGA GCAAGAAAAC TTCACCCTCG AAGTCCCAAA AGGGATGAGA
601      AAGTTTTGTG TCAAGGTGAT GCCTCACGTG GAATCCCGAA TTAACAAGGC AGAGTGGTCG
661      GAGGAGCAGT GTTTACATGT CACGACGGAG CATTATTTCA CCGTGACCAA CCTCAGCATT
721      TTCTTCTTAT CCATCCTGAT ACTCTGTGGA GCCCTGGTCT GCCTGGTTCT CCTGTGGTAC
781      ATCCGGCACC CGGGGAAGTT ACCTGCAGTC CTGGTCTTCG AGAAGCCTCC CGACTTTCTG
841      CTGGCCAACC CTCGCTGCCC AGAGACTCTC GATGACATTC ACATCCTGGA CCTGGAGGCC
901      TTCCCAAAGG TGTCACCCGA GCTGAAAGAC TCAGACCTGC ATGGCAGCAC CGACAGTGGC
961      TTTGGCAGTG GCAAACCATC ACTTCAGACG GAAGAATCCC AATTCTTCCT CCTTGACTCC
1021     CACGCCCAGA TACAGGAGAC TCTGGGAAAG GAAGGGTCTT CAGAGCTACA GGACACCTGT
1081     GGGGACAACA CGGACAGTGG GATCTGCTTG CAGGAGTCCA GCTTACACTC TAGCATGGGT
1141     CCTCCCTGGA AGCAGCAGTT CGGATATACC CACCAGGACC AGGATGACAG TGACCTAGTC
1201     CAGAACTCTC CAGGGCATCC TAAGCACACA CAGGATGGCT CCGCCTTGGG CCATGGTTGT
1261     CTCCTAGAGC CTGAAGTCCC TGAGGAGAAA GACCAAGTCA TGGTAACGTT CCAGGGCTAC
1321     CAGAAGCAGA CCAGATGGAA GGAGGAAGCA GCAGAACCTT TGGACGGAGA GATTCCCTTG
1381     GCAGAAGCCT TTGATCCTGA ACTCGGGGTG CGCCTGCAGG GTGATTCAGC TTGGCCTCCT
1441     GTGGCTCTAG CCACGGGTTA TTTGAAACAG GAGTCTCAAG GGATGGCCTC TGCTCCACCG
1501     GGGACACCAA GTAGACAGTG GAATCAACTG GCTGAGGAAT GCTCACTCCT GGGTGTGGTT
1561     AGCTGTGAAG ACCTAAGCAT AGAGAGTTTG GGTTTTGCCC ATGAACTTGT CCCTCTGGAC
1621     TGTGGGGCAG CCTCTAGTGG CCTCCTGGAT AGCCTTGGCA CTAATCTGGT CACCCTGCCA
1681     TTGATCTCTA GCCTGCAGAT AGAAGAGTGA

 

Quality Control:cDNA sequencing results is available upon request.