Rat IL10RB / IL10R2 Protein (Cytokine)

Interleukin 10 receptor, beta Protein Datasheet

IL10RB / IL-10R2 Protein Product Information

• Synonym: IL10RB
• Protein Construction: A DNA sequence encoding the rat IL10RB (Met1-Pro222) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

IL10RB / IL-10R2 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 22
• Molecular Mass: The recombinant rat IL10RB/Fc is a disulfide-linked homodimer. The reduced monomer comprises 442 amino acids and has a predicted molecular mass of 50.7 kDa. The apparent molecular mass of the protein is approximately 68 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL10RB / IL-10R2 protein

IL10RB / IL-10R2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL10RB / IL-10R2 Protein Description

The functional IL-10 receptor consists of two polypeptide chains, a ligand binding chain IL-10 Rα (IL-10 R1) and a signal transduction molecule known as IL-10 Rβ (IL-10 R2). Both chains of the IL-10 R are expressed on many hematopoietic cells, including lymphocytes, monocytes and neutrophils, with the IL-10 Rβ chain expressed at higher levels than the IL-10 Rα chain in most cases. Interleukin 10 receptor beta (IL-10 Rβ) , also known as CD210b and CRF2-4, is an 80-85 kDa member of the type I I cytokine receptor family of proteins and contains two fibronectin type-III domains. The widely expressed IL-10 Rβ serves as a signal transducing accessory chain not only for IL-10, but also for other interleukins including IL-22, -28A, -28B and -29 (plus IL-26 in human) when complexed to the ligand-binding chains. It has been shown that the action of IL-10 delivered through its receptor can affect a variety of immune functions including inhibition of pro-inflammatory cytokine synthesis and regulation of the growth and function of B cells and antigen presenting cells. The human IL-10 Rβ shares 69% homology to its mouse counterpart in amino acid sequence. Research evidences indicated that defects in IL10RB are associated with susceptibility to hepatitis B virus infection.

References

1. Liu Y. et al., 1994, J Immunol. 152:1821-9.
2. Kotenko SV. et al.,1997, EMBO J. 16 (19): 5894-903.
3. Gibbs VC. et al., 1997, Pennica Gene. 186: 97-101.
4. Kotenko SV. et al., 2001, J Biol Chem. 276: 2725-32.
5. Crepaldi L. et al., 2001, J Immunol. 167: 2312-22.
6. Dinant S. J. et al., 2007, Surg Res. 141 (2): 176-82.