Cynomolgus monkey IL10RA Gene cDNA Clone / ORF Clone (Cytokine)

IL10RA cDNA Clone Datasheet

IL10RA cDNA Clone Product Information

• cDNA Size: 1773
• Gene Synonym: IL10RA
• Source: Cynomolgus monkey

IL10RA cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) interleukin 10 receptor, alpha DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 319 T/C resulting in the amino acid Trp substitution by Arg and 771 C/T，849 A/G，1011 T/G，1074 A/G not causing the amino acid variation.
  Please check the sequence information before order.

IL10RA cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL10RA cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL10RA cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGTAGCTG TTATATTGCT TGCCACCATC CCCAGCCTCC TCCTCATCCT TGGGGGGTGG
61       GTCCTCCCAG CTGTGGTACT GACATTCTTC TCCCCAGGGA CAGAGCTGCC CAGCCCGCCA
121      TCTGTGTGGT TTGAAGCAGA ATTTTTCCAC CACATCCTCC ACTGGACACC CATCCCAAAT
181      CAGTCTGAAA GTACCTGCTA TGAAGTGGCA CTCCTGAGGT ATGGAACAGG GCGCTGGAAC
241      TCCATCTCCA ACTGTAGCCA GGCCCTGTCC TATGACCTTA CCGCGGTGAC CTTGGACCTG
301      TACCGCAGCA ATGGCTACCG GGCCAGAGTG CGTGCTGTGG ACGGCAGCCG GCACTCCAAC
361      TGGACCGTCA CCAACACCCG CTTCTCTTTG GATGAAGTGA CTCTGACAGT TGGCAGTGTG
421      AAGCTAGAGA TCCACAATGG CTTCATCCTT GGGAAGATTC AGCCCCCCAG GCCCAAGATG
481      GCTCCTGCAA ATGACACATA TGAAAGCATC TTCAGTCACT TCCGAGAGTA TGAGATTGCC
541      ATTCGCAAGG TGCCGGGAAA CTTTACGTTC ACACACAAGA AAGTAAAACA TGAAAACTTC
601      AGCCTCCTAA CCTCTGGAGA AGTGGGAGAG TTCTGTGTCC AGGTGAAACC ATCTGTCACT
661      TCCCGAACTA ACAAGGGGAT GTGGTCTAAA GAGGAGTGCG TCTCCCTCAC CAGGCAGTAT
721      TTCACCGTGA CCAACGTCAT CATCTTCTTT GCCTTTGTCC TGCTGCTCTC TGGAGCCCTG
781      GCCTACTGCC TGGCCCTCCA GCTGTATGTG CGGCGCCGAA AGAAGCTGCC CAGGGTCCTG
841      CTCTTCAAGA AGCCCAACGC CTTCATCTTC ATCAGCCAGC GTCCCTCCCC AGAGACCCAA
901      GACACCATCC ACCCGCTTGA TGAGGAGGCC TTCCTGAAGG TGTCACCAGA GCTGAGGAAC
961      TCGGACCTGC ATGGCAGCAC GGACAGTGGC TTTGGCAGTA CCAAACCATC CCTGCAGACC
1021     GAAGAGCCCC AGTTCCTCCT CCCTGACCCT CACCCCCAGG CTGACAGAAC GCTGGGAAAC
1081     GGAGAGCCCC CTGAGCTGGG CGACAGCTGC AGTAGTGGCA GCAGCAATAG CACGGACAGC
1141     GGGATCTGCC TGCAGGAGCC CAGCCTGAGC CCCAGCACTG GGCCCACCTG GGAGCAGCAG
1201     GTGGGGAGCG ACAGCAGGGG CCAGGATGAC AGTGGCATTG GCCTAGTTCA AAACTCTGAG
1261     GGCCAGGCTG GGGACACACA GGGTGGCTCA GCCTTGGGCC ACGACAGTCC CCCAGAGCCT
1321     GAGGTGCCTG CGGAACAAGA CCCAACTGCT GTGGTATTCC GGGGCTACCT GAGGCAGACC
1381     AGATGCGCTG AGGAGAAGGC AACCAAGACA GGCTGCCTGG AGGAAGAATT GCCCCTGACA
1441     GGTGGCCTTG GGCCCAAATT CAGGGGATGC CTGGATGACG AAGCAGGCTT GCATCCATCA
1501     GCCCTGGCCA AGGGCTATTT GAAACAGGAT CCCCTAGAAA TGACTCTGGC TTCCTCGGGG
1561     GCCCCAGCTG AACAGTGGAA CCAGCCCACT GAGGAATGGT CACTCCTGGC CTTGAGCAGC
1621     TGCAGTGACC TGGGAACATC TGACTGGAGC TTTGCCCATG ACCTTGCCCC TCTAGGCTGT
1681     GTGGCAGCCC CAGATGGTCT CCTGGGCAGC TTTAACTCAG ACCTGGTCAC CCTGCCCCTC
1741     ATCTCTAGCC TGCACTCGAG TGACTCGAGC TGA

 

Quality Control:cDNA sequencing results is available upon request.