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Canine IL10 Gene cDNA Clone (full-length ORF Clone)
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Canine IL10 Gene cDNA Clone (full-length ORF Clone) (Cytokine)

Catalog Vector (Price) Quantity In Stock Operation
DG70022-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

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IL10 cDNA Clone Datasheet

 

IL10 cDNA Clone Product Information

Gene Bank Ref.ID: NM_001003077.1

cDNA Size:

546

Gene Synonym:

IL-10;IL10

Source: Canine

IL10 cDNA Clone Description

cDNA Description:

ORF Clone of Canis lupus familiaris interleukin 10 DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 80 T/C, 161-162 TC/CT resulting in the amino acid Leu substitution by Pro, Ile substitution by Thr and 204 C/T,399 T/G not causing the amino acid variation.

IL10 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL10 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O Briefly centrifuge the tube for 10 seconds.
  3. Incubate the tube at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL10 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCATGGCT CAGCACTGCT CTGTTGCTGC CTGGTCCTCC TGGCCGGGGT GGGAGCCAGC
61       CGACACCAGA GCACCCTACC TGAGGACGAC TGCACCCACT TCCCAGCCAG CCTGCCCCAC
121      ATGCTCCGAG AGCTCCGAGC TGCCTTCGGG AGGGTGAAGA CTTTCTTTCA AATGAAGGAC
181      AAGCTGGACA ACATACTGCT GACTGGGTCC CTGCTGGAGG ACTTTAAGAG TTACCTGGGT
241      TGCCAAGCCC TGTCGGAGAT GATCCAGTTT TACTTGGAGG AGGTGATGCC CCGGGCTGAG
301      AACCACGACC CAGACATCAA GAACCACGTG AACTCCCTGG GAGAGAAGCT CAAGACCCTC
361      AGGCTGAGAC TGAGGCTGCG ACGCTGTCAC CGATTTCTGC CCTGTGAGAA TAAGAGCAAG
421      GCGGTGGAGC AGGTGAAGAG CGCATTTAGT AAGCTCCAGG AGAAAGGTGT CTACAAAGCC
481      ATGAGTGAGT TTGACATCTT CATCAACTAC ATAGAAACCT ACATGACAAT GAGGATGAAA
541      ATCTGA

 

Quality Control:cDNA sequencing results is available upon request.

 

IL10 / IL-10 related areas, pathways, and other information

 

All IL-10 Proteins

Product nameProduct name
IL22R Protein (His Tag) Rat IL20RB Protein (His Tag)
IL20Rα Protein (His Tag)IL20 / Interleukin-20 Protein (Native)
IL10Rb ProteinRat IL10RB / IL10R2 Protein
IL22BP / IL22RA2 Protein (His Tag)Rat IL20RB Protein
IL10Rb Protein (His Tag)Cynomolgus IL10RA Protein
IL22BP / IL22RA2 ProteinRat IL20 / Interleukin-20 Protein (Native)
IL10 / Interleukin-10 Protein (His Tag)IL22 / IL-22 / Interleukin 22 Protein (Native)
IL28B / IL28C / Interleukin-28B Protein (His Tag)IL10 / Interleukin-10 Protein (Native)
IL-29 / Interleukin-29 Protein (His Tag)Canine IL22RA1 / IL22R Protein
IL-24 / Interleukin-24 Protein (His Tag)Canine IL22RA1 / IL22R Protein (His Tag)
Mouse IL10RB / IL10R2 ProteinSus scrofa (Pig) IL10 / Interleukin-10 Protein (Native)
Mouse IL10RB / IL10R2 Protein (His Tag)Feline IL10 / Interleukin-10 Protein (Native)

All IL-10 Antibodies

Product nameProduct name
IL20RA / IL20R1 Antibody (Mouse Monoclonal Antibody) IL22RA2 / IL22BP Antibody
IL20RA / IL20R1 AntibodyIL22RA2 / IL22BP Antibody
IL20RA / IL20R1 Antibody (Antigen Affinity Purified)IL22RA2 / IL22BP Antibody
IL22RA2 / IL22BP AntibodyIL22RA2 / IL22BP Antibody
IL10RB / CRFB4 AntibodyIL-28B / IFN-lambda-3 Antibody (Antigen Affinity Purified)
IL10RB / CRFB4 Antibody (Antigen Affinity Purified)IL20RA / IL-20RA Antibody
IL10RB / CRFB4 Antibody (Mouse Monoclonal Antibody)IL20RA / IL-20RA Antibody
IL22RA2 / IL22BP Antibody (Antigen Affinity Purified)IL10RB / IL10R2 Antibody (Antigen Affinity Purified)
IL20RA / IL20R1 Antibody (Rabbit Monoclonal Antibody)IL-28B / IFN-lambda-3 Antibody
IL20RA / IL20R1 Antibody (Rabbit Monoclonal Antibody)SIL10RB / IL10R2 Antibody
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"