|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Canis lupus familiaris interleukin 10 DNA.|
|Identical with the Gene Bank Ref. ID sequence except for the point mutations: 80 T/C, 161-162 TC/CT resulting in the amino acid Leu substitution by Pro, Ile substitution by Thr and 204 C/T，399 T/G not causing the amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Canine IL10 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||DG70022-G-F|
|Canine IL10 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||DG70022-G-H|
|Canine IL10 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||DG70022-G-M|
|Canine IL10 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||DG70022-G-N|
|Canine IL10 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||DG70022-G-Y|
|Product name||Product name|
IL-10 is a anti-inflammatory cytokine which belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.