Canine IL10 Gene cDNA Clone / ORF Clone (Cytokine)

Catalog	Vector (Price)	Quantity	In Stock	Operation
DG70022-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

IL10 cDNA Clone Datasheet

IL10 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_001003077.1
cDNA Size:	546
Gene Synonym:	IL-10;IL10
Source:	Canine

IL10 cDNA Clone Description

cDNA Description:	ORF Clone of Canis lupus familiaris interleukin 10 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 80 T/C, 161-162 TC/CT resulting in the amino acid Leu substitution by Pro, Ile substitution by Thr and 204 C/T，399 T/G not causing the amino acid variation.

IL10 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

IL10 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

IL10 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCATGGCT CAGCACTGCT CTGTTGCTGC CTGGTCCTCC TGGCCGGGGT GGGAGCCAGC
61       CGACACCAGA GCACCCTACC TGAGGACGAC TGCACCCACT TCCCAGCCAG CCTGCCCCAC
121      ATGCTCCGAG AGCTCCGAGC TGCCTTCGGG AGGGTGAAGA CTTTCTTTCA AATGAAGGAC
181      AAGCTGGACA ACATACTGCT GACTGGGTCC CTGCTGGAGG ACTTTAAGAG TTACCTGGGT
241      TGCCAAGCCC TGTCGGAGAT GATCCAGTTT TACTTGGAGG AGGTGATGCC CCGGGCTGAG
301      AACCACGACC CAGACATCAA GAACCACGTG AACTCCCTGG GAGAGAAGCT CAAGACCCTC
361      AGGCTGAGAC TGAGGCTGCG ACGCTGTCAC CGATTTCTGC CCTGTGAGAA TAAGAGCAAG
421      GCGGTGGAGC AGGTGAAGAG CGCATTTAGT AAGCTCCAGG AGAAAGGTGT CTACAAAGCC
481      ATGAGTGAGT TTGACATCTT CATCAACTAC ATAGAAACCT ACATGACAAT GAGGATGAAA
541      ATCTGA

Quality Control:cDNA sequencing results is available upon request.

IL10 / IL-10 related areas, pathways, and other information

Cytokine