IL-9 Antibody (Antigen Affinity Purified)

IL-9 Antibody ( Antigen Affinity Purified ) Datasheet

IL-9 Antibody Product Information

• Immunogen: Recombinant Human IL-9 protein (Catalog#11844-H08B)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human IL-9 (rh IL-9; Catalog#11844-H08B; NP_000581.1; Gln 19-Il4144). IL-9 specific IgG was purified by Human IL-9 affinity chromatography.

IL-9 Antibody Usage Guide

• Specificity: Human IL-9
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL9 in WB. Using a DAB detection system, the detection limit for Human IL9 is approximately 8 ng/lane under non-reducing conditions and 2 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL9. The detection limit for Human IL9 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL-9 Antibody Background

Interleukin-9, also known as Cytokine P40, T-cell growth factor P40, IL-9 and IL9, is a secreted protein which belongs to theIL-7 / IL-9 family. Interleukin-9 / IL-9 is a cytokine produced by T-cells and specifically by CD4+ helper cells that acts as a regulator of a variety of hematopoietic cells. Interleukin-9 / IL-9 stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. It is a T (H)2 cell-derived cytokine that might be involved in the pathophysiology of allergic diseases. IL-9 is specifically upregulated after local allergen challenge in the lungs of atopic asthmatic patients. Lymphocytes are the major cellular source of IL-9. The increased expression and its correlation with eosinophil numbers suggest a potential role for IL-9 in the late phase of the allergic response.

References

1. Renauld JC, et al., 1990, J. Immunol. 144 (11): 4235-41.
2. Lejeune D, et al., 2001, Biochem. J. 353 (Pt 1): 109-116.
3. Toda M, et al., 2002, J. Allergy Clin. Immunol. 109 (2): 246-50.
4. Erpenbeck,V.J. et al., 2003, J Allergy Clin Immunol. 111 (6): 1319-27.
5. Knoops L, et al., 2005, Growth Factors. 22 (4): 207-15.