Canine IL-8 / CXCL8 Protein (Native) (Cytokine)

Interleukin 8 Protein Datasheet

IL-8 / CXCL8 Protein Product Information

• Synonym: IL8, CXCL8
• Protein Construction: A DNA sequence encoding the canine IL8 (P41324) (Ala23-Pro101) was expressed, with a N-terminal Met.
• Source: Canine
• Expression Host: E.coli

IL-8 / CXCL8 Protein QC Testing

• Purity: >95% as determined by SDS-PAGE
• Endotoxin: Please contact us for more information.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 23
• Molecular Mass: The recombinant canine IL8 comprises 80 amino acids and has a predicted molecular mass of 9.2 kDa. The apparent molecular mass of the protein is approximately 9 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS 0.5mMTCEP PH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: IL-8 / CXCL8 protein

IL-8 / CXCL8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

IL-8 / CXCL8 Protein Description

Interleukin-8 (IL8), also known as CXCL8, is a member of the CXC chemokine family of small (8–10 kDa) proteins, a subfamily of chemokines within the cytokine superfamily. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli (IL-1, TNF, LPS, etc). IL8 has the N-terminal tripeptide Glu-Leu-Arg (ELR) motif, which is known to mediate IL8 receptor binding and subsequent signal transduction, such as the TRAF6- NF-κB pathway, and involved in inflammatory and allergic responses. Furthermore, it has been shown that IL8 also plays a vital role in angiogenesis which is important for tumorigenesis, progression and metastasis, and accordingly the expression levels of IL8 correlate with disease progression in several human carcinomas. Multiple N-terminal processed forms for this protein are available, and this purified IL8 (6-77 amino acids) is the most prominent one.

References

1. Baqqiolini M. et al., 1992, FEBS Lett. 307: 97-101.
2. Koch A.E. et al., 1992, Science. 258: 1798-1801.
3. Bar-Eli M. et al., 1999, Pathobiology. 67: 12-8.
4. Charalambous C. et al., 2005, Cancer. Res. 65: 10347-54.
5. Manna SK. et al., 2005, J Biol Chem. 280: 7010-21.