|Block||This IL6R Neutralizing Antibody can not block the binding of recombinant IL6 (Catalog # 10395-HNAE) to its recombinant receptor IL6 Rα (Catalog # 10398-H08H) .|
|Neutralization||The neutralization activity of IL6R Neutralizing Antibody is measured by its ability to neutralize IL6 Rα-mediated inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Titer (IC50) is typically 0.15-0.6 µg/mL in the presence of 50 ng/mL Recombinant Human IL6 Rα (Catalog # 10398-H08H) and 100 ng/mL Recombinant Human IL6 (Catalog # 10395-HNAE) .|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-IL-6R Antibody (APC)||Rabbit||Monoclonal||FCM||50280-R033-A|
|Anti-IL-6R Antibody (PE)||Rabbit||Monoclonal||FCM||50280-R033-P|