Anti-IL-6R Antibody

Cat: 50280-RP02
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All IL-6R Reagents
Anti-IL-6R Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-IL-6R Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Mouse
Specificity
Mouse IL-6R
Immunogen
Recombinant Mouse IL6RA protein (Catalog#50280-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse IL6RA / CD126 (rM IL6RA; Catalog#50280-M08H; NP_034689.2; Ile 44-Glu 357). IL6RA specific IgG was purified by mouse IL6RA affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IL-6R Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IL-6R Antibody (Rabbit Polyclonal antibody) Images
Mouse IL6R/IL-6R/CD126 Western blot (WB) 13660

Anti-IL6RA rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lane B: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:52 kDa

Observed band size:65 kDa

(We are unsure as to the identity of these extra bands.)

IL-6R Background Information

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
interleukin 6 receptor
References
  • Barill S, et al. (2000) The role of interleukin-6 and interleukin-6/interleukin-6 receptor-alpha complex in the pathogenesis of multiple myeloma. Eur Cytokine Netw. 11(4): 546-51.
  • Kang KW, et al. (2007) Novel role of IL-6/SIL-6R signaling in the expression of inducible nitric oxide synthase (iNOS) in murine B16, metastatic melanoma clone F10.9, cells. Free Radic Biol Med. 42(2): 215-27.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IL-6R Antibody Rabbit Polyclonal WB,ELISA 81072-T48
    Anti-IL-6R Antibody (APC) Rabbit Monoclonal FCM 50280-R033-A
    Anti-IL-6R Antibody (PE) Rabbit Monoclonal FCM 50280-R033-P
    Anti-IL-6R Antibody Rabbit Monoclonal ELISA(Cap),FCM,ICC/IF,IF 50280-R033
    Anti-IL-6R Antibody Rabbit Polyclonal ELISA 50280-RP01
    Anti-IL-6R Antibody Rabbit Monoclonal ELISA 50280-R005
    Anti-IL-6R Antibody Rabbit Polyclonal ELISA 10398-RP02
    Anti-IL-6R Antibody Rabbit Polyclonal ELISA 10398-RP03
    Anti-IL-6R Antibody Rabbit Polyclonal ELISA 10398-RP01
    Anti-IL-6R Antibody Mouse Monoclonal ELISA 10398-MM01
    Anti-IL-6R Antibody Rabbit Polyclonal ELISA 10398-T16
    Anti-IL-6R Antibody Mouse Monoclonal ELISA(Cap),ICC/IF,IF 10398-MM18
    Anti-IL-6R Antibody Mouse Monoclonal Neutralization 10398-MM08
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