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IL-6R / CD126 Antibody, Mouse MAb

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Human IL6R Antibody Product Information
Immunogen:Recombinant Human IL-6R / CD126 protein (Catalog#10398-H08H)
Clone ID:18
Ig Type:Mouse IgG1
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-6R / CD126 (rh IL-6R / CD126; Catalog#10398-H08H; NP_000556.1; Met1-Pro365). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Human IL6R Antibody Usage Guide
Specificity:Human IL-6R / CD126
Application:ICC/IF, IF

ICC/IF: 10-25 μg/mL

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human IL6R Antibody IF Application Image
IL-6R / CD126 Antibody, Mouse MAb, Immunofluorescence
[Click to enlarge image]
Caption:
Immunofluorescence staining of Human IL6R in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IL6R monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Other IL6R Antibody Products
Reactivity: Human  
Application: B/N  
  • Slide 1
10398-MM08-500
10398-MM08-200
500 µg 
200 µg 
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Reactivity: Human  
Application: ELISA  
    10398-MM01-50
    10398-MM01-100
    10398-MM01-200
    50 µg 
    100 µg 
    200 µg 
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    Reactivity: Human  
    Application: ELISA  
      10398-RP02-400
      10398-RP02-100
      10398-RP02-200
       
      100 µg 
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      Reactivity: Human  
      Application: ELISA  
        10398-RP03-200
        10398-RP03-50
        10398-RP03-100
        200 µg 
        50 µg 
        100 µg 
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        Reactivity: Human  
        Application: ELISA  
          10398-T16-200
          10398-T16-50
          10398-T16-100
          200 µg 
          50 µg 
          100 µg 
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          Reactivity: Human  
          Application: ELISA  
            10398-RP01-400
            10398-RP01-200
            10398-RP01-100
             
            200 µg 
            100 µg 
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            Reactivity: Mouse  
            Application: ELISA  
              50280-RP01-100
              50280-RP01-200
              50280-RP01-400
              100 µg 
              200 µg 
               
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              Reactivity: Mouse  
              Application: ELISA  WB  
              • Slide 1
              50280-RP02-50
              50280-RP02-100
              50280-RP02-200
              50 µg 
              100 µg 
              200 µg 
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              Reactivity: Mouse  
              Application: ELISA  
                50280-R005-50
                50280-R005-100
                50 µg 
                100 µg 
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                Reactivity: Mouse  
                Application: FCM  
                • Slide 1
                50280-R033-P-100
                50280-R033-P-50
                100 Tests 
                50 Tests 
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                Reactivity: Mouse  
                Application: FCM  
                • Slide 1
                50280-R033-A-100
                50280-R033-A-50
                100 Tests 
                50 Tests 
                Add to Cart
                Reactivity: Mouse  
                Application: FCM  IF  ICC/IF  
                • Slide 1
                • Slide 1
                50280-R033-100
                50280-R033-50
                100 µg 
                50 µg 
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                Reactivity: Rat  
                Application: ELISA  WB  
                • Slide 1
                81072-T48-50
                81072-T48-100
                81072-T48-200
                50 µg 
                100 µg 
                200 µg 
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                IL6R/IL-6R/CD126 Background

                Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

                Human IL6R/IL-6R/CD126 References
              • Barill S, et al. (2000) The role of interleukin-6 and interleukin-6/interleukin-6 receptor-alpha complex in the pathogenesis of multiple myeloma. Eur Cytokine Netw. 11(4): 546-51.
              • Kang KW, et al. (2007) Novel role of IL-6/SIL-6R signaling in the expression of inducible nitric oxide synthase (iNOS) in murine B16, metastatic melanoma clone F10.9, cells. Free Radic Biol Med. 42(2): 215-27.
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                Size / Price
                Catalog: 10398-MM18-200
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                All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"