Anti-IL-6R Antibody

Cat: 10398-MM18
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Anti-IL-6R Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-IL-6R Antibody
Validated applications
ELISA(Cap),ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human IL-6R
Immunogen
Recombinant Human IL-6R / CD126 protein (Catalog#10398-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-6R / CD126 (rh IL-6R / CD126; Catalog#10398-H08H; NP_000556.1; Met1-Pro365). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #18
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IL-6R Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ICC-IF 1:20-1:100  
ELISA(Cap) 1:250-1:2000 This antibody will detect Human IL6R / IL-6R / CD126 in ELISA pair set (Catalog: # SEKA10398). In a sandwich ELISA, it can be used as capture antibody.

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IL-6R Antibody (Mouse Monoclonal antibody) Images
Human IL6R/IL-6R/CD126 Immunofluorescence(IF) 2903
Immunofluorescence staining of Human IL6R in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human IL6R monoclonal antibody (1:60) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to plasma membrane.
Anti-IL-6R Antibody Alternative Names
Anti-CD126 Antibody;Anti-gp80 Antibody;Anti-IL-6R Antibody;Anti-IL-6R-1 Antibody;Anti-IL-6RA Antibody;Anti-IL6Q Antibody;Anti-IL6RA Antibody;Anti-IL6RQ Antibody
IL-6R Background Information

Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (sIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a sIL-6R protein are able to mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signalling. Through this so-called 'trans-signalling' mechanism, IL-6 is able to stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and sIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.

Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
interleukin 6 receptor
References
  • Barill S, et al. (2000) The role of interleukin-6 and interleukin-6/interleukin-6 receptor-alpha complex in the pathogenesis of multiple myeloma. Eur Cytokine Netw. 11(4): 546-51.
  • Kang KW, et al. (2007) Novel role of IL-6/SIL-6R signaling in the expression of inducible nitric oxide synthase (iNOS) in murine B16, metastatic melanoma clone F10.9, cells. Free Radic Biol Med. 42(2): 215-27.
  • Product Description Host Clonality Application Catalog# (PDF)
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    Anti-IL-6R Antibody Mouse Monoclonal ELISA 10398-MM01
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