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pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.
The coding sequence can be amplified by PCR with M13-47 and RV-M primers.
|Human IL6 Gene cDNA Clone (full-length ORF Clone) expression ready, FLAG-tagged||HG10395-M-F|
|Human IL6 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||HG10395-M-H|
|Human IL6 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||HG10395-M-M|
|Human IL6 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||HG10395-M-N|
|Human IL6 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||HG10395-M-Y|
Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.