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Human IL6 Gene cDNA Clone (full-length ORF Clone)

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IL6cDNA Clone Product Information
Gene Bank Ref.ID:NM_000600.2
cDNA Size:639
cDNA Description:ORF Clone of Homo sapiens interleukin 6 (interferon, beta 2) DNA.
Gene Synonym:HGF, HSF, BSF2, IL-6, IFNB2, IL6
Species:Human
Vector:pMD18-T Simple Vector
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Shipping Carrier:Each tube contains approximately 10 μg of lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at ambient temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
IL-6 Family & Receptor Related Products
Product nameProduct name
Human IL6ST / gp130 / CD130 Protein (His & Fc Tag)Human IL6ST / gp130 / CD130 ProteinMouse LIF Protein (Fc Tag)Mouse LIF Protein (His Tag)Human CNTF Protein (His Tag)Human GM-CSF / CSF2 Protein (Fc Tag)Human G-CSF / CSF3 Protein (Fc Tag)Human NNT1 / CLCF1 / CLC Protein (Fc Tag)Canine IL11RA / IL-11RA / IL11Rα ProteinRat CNTF / Ciliary Neurotrophic Factor ProteinRat CNTFR / CNTFR-alpha Protein (His Tag)Rat GM-CSF / CSF2 Protein (Fc Tag)Rat GM-CSF / CSF2 Protein (His Tag)Rat gp130 / IL6ST / CD130 Protein (His & Fc Tag)Rat gp130 / IL6ST / CD130 Protein (His Tag)Human CNTFR / CNTFR-alpha Protein (His Tag)Mouse Oncostatin M / OSM Protein (His Tag)Human Leptin ProteinHuman LIF Protein (His Tag)Rat IL6 / Interleukin-6 ProteinHuman LIF Protein (Fc Tag)Mouse Oncostatin M / OSM ProteinMouse CNTF / Ciliary Neurotrophic Factor Protein (His Tag)Human GM-CSF / CSF2 ProteinMouse IL11RA / IL11Rα Protein (His Tag)Human GM-CSF / CSF2 Protein (His Tag)Human GM-CSF / CSF2 ProteinHuman Oncostatin M / OSM Protein (His Tag)Mouse IL6 / Interleukin-6 ProteinRat LIFR Protein (His Tag)Human LIFR / CD118 Protein (His Tag)Human G-CSFR / CD114 / CSF3R Protein (Fc Tag)Human G-CSFR / CD114 Protein (His Tag)Human G-CSFR / CD114 / CSF3R ProteinHuman G-CSF / CSF3 Protein (isoform b)Human Oncostatin M / OSM ProteinCynomolgus / Rhesus IL6 / Interleukin-6 ProteinHuman IL11RA / IL11Rα Protein (His Tag)Mouse IL6ST / CD130 Protein (His & Fc Tag)Mouse IL6ST / gp130 / CD130 Protein (His & Fc Tag)Mouse IL6ST / gp130 / CD130 Protein (His Tag)Mouse IL-31 / IL31 Protein (His Tag)Rat IL-6R / CD126 Protein (ECD, His Tag)Canine IL11RA / IL-11RA / IL11Rα Protein (His Tag)Human IL6 / Interleukin-6 ProteinHuman IL-31 / IL31 Protein (His Tag)Human G-CSF / CSF3 Protein (isoform b)Human CSF2RA / GM-CSFR / CD116 Protein (His Tag)Human CSF2RA / GM-CSFR / CD116 Protein (Fc Tag)Mouse GM-CSF / CSF2 ProteinHuman Leptin Receptor / LEPR / CD295 Protein (His & Fc Tag)Human Leptin Receptor / LEPR / CD295 Protein (His Tag)Human LIF ProteinCynomolgus IL6ST / gp130 Protein (Fc Tag)Cynomolgus IL6ST / gp130 Protein (His Tag)Mouse IL6RA / CD126 Protein (His Tag)Mouse GM-CSF / CSF2 Protein (Fc Tag)Mouse GM-CSF / CSF2 Protein (His Tag)Human IL11 / Interleukin 11 / IL-11 ProteinHuman OSMR / IL31RB Protein (His Tag)Human IL6R / CD126 Protein (His Tag)Mouse LIFR / CD118 Protein (His Tag)Mouse OSMR / IL-31RB Protein (His Tag)
Background

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

References
  • Heinrich PC. et al. (2003). Principles of interleukin-6-type cytokine signalling and its regulation. Biochem J. 374: 1-20.
  • Rose-John S, et al. (2007) The IL-6/sIL-6R complex as a novel target for therapeutic approaches. Expert Opin Ther Targets. 11(5): 613-24.
  • Dinh W, et al. (2009) Elevated plasma levels of TNF-alpha and interleukin-6 in patients with diastolic dysfunction and glucose metabolism disorders. Cardiovasc Diabetol. 8:58.
  • Catalog:HG10395-M
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