IL-6 Antibody (Cytokine)

IL-6 Antibody Datasheet

IL-6 Antibody Product Information

• Immunogen: Recombinant Human IL-6 Protein (Catalog#10395-HNAE)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 057
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human IL-6 ( rh IL-6; Catalog#10395-HNAE; Val 30 - Met 212; NP_000591.1)

IL-6 Antibody Usage Guide

• Specificity: Human IL-6
• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human IL6 in WB. Using a DAB detection system, the detection limit for Human IL6 is approximately 0.25 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human IL6. The detection limit for Human IL6 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL-6 Antibody Background

Interleukin 6 (IL-6, IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation of various tissues, and is known particularly for its role in the immune response and acute phase reactions. IL-6/IL6 is a cytokine with a wide variety of biological functions and belongs to the IL-6 superfamily. IL-6/IL6 is a potent inducer of the acute phase response and plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. IL-6/IL6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. The role of IL-6 as an anti-inflammatory cytokine is mediated through its inhibitory effects on TNF-alpha and IL-1, and activation of IL-1Ra and IL-10. IL-6/IL6 is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6/IL6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Inhibitors of IL-6 are used to treat postmenopausal osteoporosis. IL-6/IL6 is thought to be a reason why obese individuals have higher endogeneous levels of CRP. Intranasally administered IL-6 was shown to improve sleep-associated consolidation of emotional memories.

References

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