Anti-IL-33 Antibody

Cat: 10368-MM03
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All IL-33 Reagents
Anti-IL-33 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-IL-33 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human IL-33
No cross activity in ELISA with Human cell lysate (293 cell line)
Recombinant Human IL-33 protein (Catalog#10368-HNAE)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-33 (rh IL-33; Catalog#10368-HNAE; NP_254274.1; Ser 112-Thr 270). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2b Clone #03
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IL-33 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IL-33 Antibody (Mouse Monoclonal antibody) Images
Human IL-33 Immunohistochemistry(IHC) 2904
Immunofluorescence staining of Human IL33 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human IL33 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cytoplasm.
Human IL-33 Flow Cytometry (FC) 4911
Flow cytometric analysis of Purified anti-Human IL-33 on HUVEC cells. HUVEC cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-IL-33 (Bold line hisgram), then stained with a FITC-conjugated second step antibody.
Anti-IL-33 Antibody Alternative Names
Anti-C9orf26 Antibody;Anti-DVS27 Antibody;Anti-IL1F11 Antibody;Anti-NF-HEV Antibody;Anti-NFEHEV Antibody
IL-33 Background Information

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial enules), is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

Full Name
interleukin 33
  • Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8.
  • Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IL-33 Antibody Rabbit Polyclonal ELISA 70005-T16
    Anti-IL-33 Antibody Mouse Monoclonal ELISA 70005-MM05
    Anti-IL-33 Antibody Rabbit Polyclonal WB 100330-T32
    Anti-IL-33 Antibody (PE) Rabbit Monoclonal FCM 10368-R002-P
    Anti-IL-33 Antibody Rabbit Polyclonal ELISA 10368-T16
    Anti-IL-33 Antibody Rabbit Polyclonal ELISA,IHC-P 10368-T24
    Anti-IL-33 Antibody Rabbit Monoclonal ELISA,FCM,ICC/IF,IF 10368-R002
    Anti-IL-33 Antibody (FITC) Rabbit Monoclonal FCM 10368-R002-F
    Anti-IL-33 Antibody (FITC) Mouse Monoclonal FCM 10368-MM03-F
    Anti-IL-33 Antibody Mouse Monoclonal IHC-P 10368-MM13
    Anti-IL-33 Antibody Mouse Monoclonal IHC-P 10368-MM27
    Anti-IL-33 Antibody Rabbit Polyclonal WB,ELISA 10368-RP01
    Anti-IL-33 Antibody Rabbit Polyclonal WB,ELISA 10368-RP02
    Anti-IL-33 Antibody Mouse Monoclonal ELISA 10368-MM02
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