Anti-IL-33 Antibody

Cat: 10368-MM03
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All IL-33 Reagents
Anti-IL-33 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-IL-33 Antibody
Validated applications
ELISA,FCM,ICC/IF,IF
Species reactivity
Reacts with: Human
Specificity
Human IL-33
No cross activity in ELISA with Human cell lysate (293 cell line)
Immunogen
Recombinant Human IL-33 protein (Catalog#10368-HNAE)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-33 (rh IL-33; Catalog#10368-HNAE; NP_254274.1; Ser 112-Thr 270). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG2b Clone #03
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-IL-33 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ICC-IF 1:20-1:100  
FCM 1:25-1:100  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-IL-33 Antibody (Mouse Monoclonal antibody) Images
Human IL-33 Immunohistochemistry(IHC) 2904
Immunofluorescence staining of Human IL33 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human IL33 monoclonal antibody (1:60) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green) and counterstained with DAPI(blue). Positive staining was localized to cytoplasm.
Human IL-33 Flow Cytometry (FC) 4911
Flow cytometric analysis of Purified anti-Human IL-33 on HUVEC cells. HUVEC cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-IL-33 (Bold line hisgram), then stained with a FITC-conjugated second step antibody.
Anti-IL-33 Antibody Alternative Names
Anti-C9orf26 Antibody;Anti-DVS27 Antibody;Anti-IL1F11 Antibody;Anti-NF-HEV Antibody;Anti-NFEHEV Antibody
IL-33 Background Information

Interleukin 33 (IL-33), also known as DVS27 or NF-HEV (Nuclear Factor from High Endothelial enules), is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 protein is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. IL-33 protein has transcriptional regulatory properties, and the researches suggested that IL-33 is a dual-function protein that might act both as a cytokine and as an intracellular nuclear factor. As a type 2 cytokines, IL-33 protein also play a pivotal role in helminthic infection and allergic disorders.

Full Name
interleukin 33
References
  • Iikura M, et al. (2007) IL-33 can promote survival, adhesion and cytokine production in human mast cells. Lab Invest. 87(10): 971-8.
  • Lamkanfi M, et al. (2009) IL-33 raises alarm. Immunity. 31(1): 5-7.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-IL-33 Antibody Rabbit Polyclonal ELISA 70005-T16
    Anti-IL-33 Antibody Mouse Monoclonal ELISA 70005-MM05
    Anti-IL-33 Antibody Rabbit Polyclonal WB 100330-T32
    Anti-IL-33 Antibody (PE) Rabbit Monoclonal FCM 10368-R002-P
    Anti-IL-33 Antibody Rabbit Polyclonal ELISA 10368-T16
    Anti-IL-33 Antibody Rabbit Polyclonal ELISA,IHC-P 10368-T24
    Anti-IL-33 Antibody Rabbit Monoclonal ELISA,FCM,ICC/IF,IF 10368-R002
    Anti-IL-33 Antibody (FITC) Rabbit Monoclonal FCM 10368-R002-F
    Anti-IL-33 Antibody (FITC) Mouse Monoclonal FCM 10368-MM03-F
    Anti-IL-33 Antibody Mouse Monoclonal IHC-P 10368-MM13
    Anti-IL-33 Antibody Mouse Monoclonal IHC-P 10368-MM27
    Anti-IL-33 Antibody Rabbit Polyclonal WB,ELISA 10368-RP01
    Anti-IL-33 Antibody Rabbit Polyclonal WB,ELISA 10368-RP02
    Anti-IL-33 Antibody Mouse Monoclonal ELISA 10368-MM02
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