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IL-33 Antibody, Mouse MAb
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IL-33 Antibody, Mouse MAb (Cytokine) PDF Download

Catalog   Size (Price) Quantity In Stock Operation
10368-MM03  

YES   
 

Primary antibody | Secondary antibody | Tag antibody | Isotype control antibody | Loading control antibody | Antibody Purification

IL-33 Antibody Datasheet

  Order or Inquire for IL-33 Antibody product Quality antibodies Antibody production services
  Detection limit is 0.625 ng/well in ELISA
 

IL-33 Antibody Product Information

Immunogen :

Recombinant Human IL-33 protein (Catalog#10368-HNAE)

Antibody Type : Mouse Monoclonal Antibody ( Mouse mAb Service Platform )

Clone ID :

03

Ig Type :

Mouse IgG2b

Formulation : 0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.
Preparation :

This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human IL-33 (rh IL-33; Catalog#10368-HNAE; NP_254274.1; Ser 112-Thr 270). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

IL-33 Antibody Usage Guide

Specificity :

Human IL-33

  No cross-reactivity in ELISA with

Human cell lysate (293 cell line)

Flow Cytometry :
IL-33 Flow Cytometry

Flow cytometric analysis of Purified anti-Human IL-33 on HUVEC cells. HUVEC cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), and stained with Purified Mouse anti-IL-33 (Bold line hisgram, 1 μg/test), then stained with a FITC-conjugated second step antibody.

Flow cytometry was performed on a BD FACSCalibur flow cytometry system

Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

Direct ELISA : This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human IL33. The detection limit for Human IL33 is approximately 0.625 ng/well.
Storage : This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

IL-33 Antibody Related Products & Topics

IL-33 Antibody Related Areas:

Immunology>>Adaptive Immunity>>T Cell>>Helper T Cell>>IL-33

Immunology>>Cytokine & Receptor>>Interleukin & Receptor >>IL-1 superfamily and IL-1R/TLR family>>IL-33

Proteins:

Molecule Species Description //For Detailed Info. and Price------CLICK! Cat. No
IL-33 Human IL-33/NF-HEV Protein, Recombinant 10368-HNAE
IL-33 Canine IL33 / Interleukin-33 / NF-HEV Protein, Recombinant, with Native 70005-DNAE

Antibodies:

Molecule Application Description //For Detailed Info. and Price------CLICK! Cat. No
Human
IL-33
WB Mouse Monoclonal Antibody 10368-MM01
Human
IL-33
ELISA Mouse Monoclonal Antibody 10368-MM02
Human
IL-33
ELISA, FCM IL-33 Antibody 10368-MM03
Human
IL-33
FCM IL-33 Antibody ( FITC ) 10368-MM03-F
Human
IL-33
IHC-P IL-33 Antibody 10368-MM13
Human
IL-33
WB, ELISA Rabbit Polyclonal Antibody 10368-RP01
Human
IL-33
WB, ELISA Rabbit Polyclonal Antibody (Antigen Affinity Purified) 10368-RP02
Human
IL-33
ELISA, FCM IL-33 Antibody 10368-R002

IL-33 Antibody Background

Interleukin-33 (IL-33), initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules, also known as DVS 27, is a proinflammatory protein and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is expressed highly and rather selectively by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T (H)2-associated cytokines from in vitro polarized T (H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs.

References

  1. Cayrol C,Girard JP, 2009, Proc Natl Acad Sci U S A. 106 (22): 9021-6.
  2. Carriere V, et al., 2007, Proc Natl Acad Sci U S A. 104 (1): 282-7.
  3. Baekkevold, E.S. et al., 2003, Am. J. Pathol. 163:69.
  4. Schmitz, J. et al., 2005, Immunity. 23:479.
  5. Black, R.A. et al., 1989, J. Biol. Chem. 264: 5323.
  6. Xu, D. et al., 1998, J. Exp. Med. 187: 787.
  7. Dinarello, C.A., 2005, Immunity. 23: 461.
 

 

Primary Antibody Products

All IL-1 Proteins

Product nameProduct name
IL18 Protein (Native)IL-1 alpha / IL1A / IL1F1 Protein
IL18BPa ProteinMouse IL1R1 / CD121a Protein (His Tag)
IL18BPa Protein (His Tag)Cynomolgus IL-1 beta / IL1B Protein (Native)
IL1F5 / IL36RN Protein (Native)IL36B / IL1F8 Protein (Native)
IL1R2 / CD121b ProteinIL1F6 / IL36A Protein (Native)
IL1RL1 / DER4 ProteinMouse IL-1R8 / IL1RAPL1 Protein
IL1RL1 / DER4 ProteinCanine IL-1 beta / IL1B Protein (Native)
IL1R9 ProteinIL36G / IL1F9 Protein (Native)
IL1RA / IL1F3 ProteinMouse IL1F8 / IL36b Protein Native
IL1RA / IL1F3 Protein (Native)IL37 / IL1F7 / IL-1H4 Protein Native
IL-1 alpha / IL1A / IL1F1 ProteinMouse IL1R1 / CD121a Protein
IL33 / Interleukin-33 / NF-HEV Protein (Native)Canine IL33 / Interleukin-33 / NF-HEV Protein (Native)
Mouse IL-18R1 ProteinIL36B / IL1F8 Protein (His Tag)
IL-1R8 ProteinIL1F6 / IL36A Protein (His Tag)
Mouse SIGIRR ProteinRat IL18R1 Protein
IL1R8 Protein (His Tag)Rat IL1R2 / IL1RB / CD121b Protein (His Tag)
IL1R3 ProteinIL36G / IL1F9 (aa 18-169) Protein (His Tag)
IL1R1 / CD121a ProteinIL36G / IL1F9 (aa 18-169) Protein
IL1R1 / CD121a Protein (His Tag)IL1F6 / IL36 (aa 6-158) Protein
IL-1 beta / IL1B Protein (Native)Mouse IL-18 Protein (Native)
Mouse IL18BP Protein (His Tag)Mouse IL1R2 / CD121b Protein
IL18RAP / IL1R7 ProteinMouse IL1R2 / CD121b Protein (His Tag)
IL18R1 / CD218a Protein (His Tag)Rat IL1RN / IL1RA Protein
IL1R3 / IL1RAP Protein (His Tag)Cynomolgus IL18RAP Protein
IL18R1 / CD218a ProteinCynomolgus IL18RAP Protein (His Tag)
IL1RL1 / ST2 Protein (His Tag)Rat IL1R2 / IL1RB / CD121b Protein
IL1R2 / IL1RB / CD121b Protein (His Tag)Cynomolgus IL1R2 / IL1RB Protein
Mouse IL18R1 / CD218a Protein (His Tag)Mouse IL18 / IL-18 Protein
IL18RAP / IL1R7 Protein (His Tag)IL18 / Interleukin 18 / IGIF Protein (GST Tag)
Mouse IL-1 alpha / IL1A / IL1F1 Protein (Native)IL1R1 / CD121a Protein
Mouse IL1F5 / IL1RP3 Protein (Native)IL1R2 / IL1RB / CD121b Protein
IL1R9 / IL1RAPL2 / IL1 Receptor 9 Protein (His Tag)SIGIRR / TIR8 Protein
Rat IL-1 beta / IL1B ( pro form ) Protein (His Tag)Cynomolgus IL1R1 Protein
SIGIRR / TIR8 Protein (His Tag)Cynomolgus IL1R1 Protein (His Tag)
Cynomolgus IL-18 / IL-1F4 Protein (His Tag)Canine IL18R1 Protein
Mouse IL-1F6 / IL-1 epsilon Protein (Native)Canine IL1R2 / IL1RB Protein
IL-1 beta / IL1B (pro form) Protein (His Tag)Rat IL18R1 Protein (His Tag)
Rat IL1R1 / CD121a Protein (His Tag)Mouse IL36G / IL1F9 Protein (His Tag)
Mouse IL-1 beta / IL1B Protein (Native)Cynomolgus IL18R1 Protein
Rat IL1R1 / CD121a ProteinRabbit IL-1 beta / IL1B Protein
IL1RL1 / ST2 (isoform A) Protein (His Tag)Cynomolgus IL18R1 Protein (His Tag)
Rat IL-1 beta / IL1B (mature form) Protein (Native) 

All IL-1 Antibodies

Product nameProduct name
IL1RL1 / ST2 Antibody (Mouse Monoclonal Antibody)IL-18 Antibody (Antigen Affinity Purified)
IL1RL1 / ST2 AntibodySIGIRR / TIR8 Antibody
IL33 / Interleukin-33 / NF-HEV Antibody (Mouse Monoclonal Antibody)SIGIRR / TIR8 Antibody (Antigen Affinity Purified)
IL33 / Interleukin-33 / NF-HEV AntibodyIL18 / Interleukin-18 Antibody
IL18BP / IL18BPa Antibody (Mouse Monoclonal Antibody)IL-1RA Antibody
IL18BP / IL18BPa AntibodyIL-1R8 / IL1RAPL1 Antibody
IL18BP / IL18BPa AntibodyIL-1R2 / CD121b Antibody
IL18BP / IL-18BP AntibodyIL-1R9 / IL1RAPL2 Antibody (Antigen Affinity Purified)
IL18BP / IL18BPa Antibody (Antigen Affinity Purified)IL-1 beta / IL1B Antibody (Antigen Affinity Purified)
IL1R1 / CD121a AntibodyIL-1F6 Antibody (Antigen Affinity Purified)
IL1R1 / CD121a Antibody (Antigen Affinity Purified)IL-1R1 / CD121a Antibody (Antigen Affinity Purified)
IL1RL1 / ST2 Antibody (Antigen Affinity Purified)IL-1 beta / IL1B Antibody (Antigen Affinity Purified)
IL18BP / IL-18BP Antibody (Antigen Affinity Purified)IL-33 Antibody
IL1R1 / CD121a Antibody (Mouse Monoclonal Antibody)IL-1RA Antibody (FITC)
IL18R1 / CD218a AntibodyIL-1 alpha / IL-1F1 / IL1A Antibody (Antigen Affinity Purified)
IL18R1 / CD218a Antibody (Antigen Affinity Purified)IL18BP / IL18BPa Antibody
IL33 / Interleukin-33 / NF-HEV AntibodyIL1F6 / IL36A Antibody
IL33 / Interleukin-33 / NF-HEV Antibody (Antigen Affinity Purified)IL-1 alpha / IL-1F1 / IL1A Antibody
IL1R3 / IL1RAP AntibodyIL-1R1 / CD121a Antibody
IL1R2 / IL1RB / CD121b AntibodyIL-33 Antibody
IL1R2 / IL1RB / CD121b Antibody (Antigen Affinity Purified)IL-33 Antibody
IL-1 beta / IL1B / IL1F2 AntibodyIL-33 Antibody (FITC)
IL-1 beta / IL1B / IL1F2 Antibody (Antigen Affinity Purified)IL1F6 / IL36A Antibody
IL1R1 / CD121a AntibodyIL-18 Antibody
IL18R1 / IL1RRP / CD218a AntibodyIL37 / IL1F7 / IL-1H4 Antibody
IL18R1 / IL1RRP / CD218a Antibody (Antigen Affinity Purified)IL-1F5 Antibody
IL18R1 / CD218a AntibodyIL-1RAcP / IL-1R3 Antibody (FITC)
IL18BP / IL-18BP AntibodyIL-1RAcP / IL-1R3 Antibody (PE)
IL18BP / IL-18BP AntibodyIL-1RA Antibody (PE)
IL1R2 / IL1RB / CD121b AntibodyIL-33 Antibody (PE)
IL1R2 / IL1RB / CD121b AntibodyIL-33 Antibody (FITC)
IL1R2 / IL1RB / CD121b AntibodySIGIRR / TIR8 Antibody
IL18R1 / CD218a AntibodyIL1R1 / CD121a Antibody
IL1RA / IL1F3 AntibodyIL1R1 / CD121a Antibody
IL-1 beta / IL1B / IL1F2 AntibodyIL-1 beta / IL1B Antibody
IL18R1 AntibodyIL-1 beta / IL1B Antibody
IL1RL1 AntibodyIL-1R1 / CD121a Antibody
IL1RL1 AntibodyIL-1R1 / CD121a Antibody
IL-1RAcP / IL-1R3 Antibody (Antigen Affinity Purified)IL-1RA Antibody (Antigen Affinity Purified)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"